The extracellular matrix ECM) is the material found in the extracellular environment of all tissues and organs. The composition of the extracellular framework of all vertebrates is dominated by a class of molecules known as collagens, each with unique features suited for a particular function and location.
Collagen proteins are composed of three subunit polypeptides that vary in length and interact to form a triple helix due to a unique repeated (Gly-x-y) sequence. Collagen I is one of the most common forms and is found in skin, tendon, vascular ligature, organs, and bone. Because of its basic and fundamental role in ECM architecture, Collagen I antibodies are helpful in identifying and/or verifying tissue-specific origin and sample tissue typing. Collagen I antibodies have been used to assess fibroblast primary sources in pulmonary fibrosis (1). Studies looking at transcriptional regulation of Procollagen Lysyl Hydroxylase 2 (PLOD2) and Hypoxia-Inducible Factor 1 (HIF-1) in breast cancer metastasis relied upon Collagen I antibodies (2,3). Collagen I antibodies helped monitor murine spleen cell differentiation in serum-free culturing conditions (4). Wound healing studies looking at liver regeneration also relied upon Collagen I antibodies (5).
Novus Biologicals is proud to offer a wide selection of Collagen reagents including antibodies, lysates, proteins, RNAi and more!
