Monocyte Chemoattractant Protein (MCP1) is a potent monocyte attractant, is a member of the CC chemokine subfamily. MCP1 exerts its effects through binding to G-protein-coupled receptors on the surface of leukocytes targeted for activation and migration. The role of MCP1 and its receptor, chemokine receptor-2 (CCR2), in monocyte recruitment during infection or under other inflammatory conditions is well known. Recent studies indicate that MCP-1 may play an important role in pulmonary inflammation. In vitro studies show that a number of cell types are capable of producing MCP1 (1). Rat macrophage subpopulations were examined by immunohistochemistry using various anti-rat macrophage monoclonal antibodies. These studies revealed that the main sources of MCP-1 production were alveolar and interstitial macrophages and polymorphonuclear leukocytes in the rat model of bleomycin-induced lung injury (2).
MCP1 and CCR2 also play a major role in the recruitment of inflammatory cells to the lungs of patients with chronic obstructive pulmonary disease (COPD). Increased levels of MCP1 have been detected by IHC in human prostate cancer tissues by anti MCP-1 antibodies. Higher MCP1 levels were also detected by ELISA in various prostate cancer cells lines using antibodies against MCP1 suggesting that MCP1 is a key player in the growth and invasion of prostate and several cancers where MCP-1 acts as both a paracrine and autocrine factor (3). MCP1 mediated interactions between normal and malignant cells in the tumor microenvironment plays a multi-faceted role in tumor progression and could be a potential therapeutic target. Novus Biologicals offers a wide range of products and tools for your research, including cell lysates, recombinant proteins and antibodies against MCP-1 with reactivity against several species.
Novus Biologicals offers MCP1 reagents for your research needs including:
