Inositol Requiring Enzyme 1 alpha (IRE1 alpha) is a transmembrane-RNase with an endoplasmic reticulum (ER) luminal sensor domain and cytosolic kinase and ribonuclease domains. IRE1 also plays a central role in the ER stress response (1). In a recent study the function of nitric oxide (NO) in endoplasmic reticulum (ER)-related cell death in human glioma cells was investigated by treating these cells with a NO donor S-nitroso-N-acetyl-d,l-penicillamine and thapsigargin, an ER stress inducer, resulting in cell death. Expression of the ER-associated molecules IRE1 alpha was elevated as detected by Western blotting (2) suggesting IRE1 alpha may play a role in the ER related cell death. Activated IRE1 and ER stress in various cells plays an important role in the pathogenesis of several diseases, including obesity, type 2 diabetes, cancer, and intestinal bowel and airway diseases suggesting that ER stress-induced inflammation contributes substantially to disease progression (3). Targeting IRE1 may contribute towards a better therapeutic regimen for these diseases.
Westerm Blot: IRE-1 alpha antibody [NB100-2323
Novus Biologicals offers IRE1 alpha reagents for your research needs including:
