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Isgylation Bioinformatics

Protein Isgylation has been observed in Virus Diseases, Innate Immune Response, Neoplasms, Infective Disorder, Malignant Neoplasms. The study of protein Isgylation has been mentioned in research publications which can be found using our bioinformatics tool below. Related pathways to protein Isgylation have been researched including Conjugation, Immune Response, Innate Immune Response, Translation, Cell Motility. Protein Isgylation products complement our catalog of research reagents including antibodies and ELISA kits against ALDH9A1, BCHE, DBT, DLD, IFNA1.

Top Research Reagents

We have 1755 products for the study of Isgylation that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Dihydrolipoamide Dehydrogenase/DLD Antibody
NBP1-31302
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLD protein stained by DLD antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

ISG15 Activating Enzyme/UBE1L Antibody
NBP1-32579
Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579] - (30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000Immunohistochemistry-Paraffin: ISG15 Activating Enzyme/UBE1L Antibody [NBP1-32579] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

UBE2N/Ubc13 Antibody - BSA Free
NBP1-76593
Western Blot: UBE2N/Ubc13 Antibody [NBP1-76593] - Human small intestine cell lysates with Ube2N antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.Immunohistochemistry: UBE2N/Ubc13 Antibody [NBP1-76593] - Analysis of UBC13 in mouse thymus tissue with UBC13 antibody at 2.5 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

2 Publications
ALDH9A1 Antibody
NBP1-89150
Western Blot: ALDH9A1 Antibody [NBP1-89150] - Analysis in rat cell line NBT-II.Immunohistochemistry-Paraffin: ALDH9A1 Antibody [NBP1-89150] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Rabbit Polyclonal
Species Human, Rat
Applications WB, IHC, IHC-P

3 Publications
DBT Antibody
NBP1-89522
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Analysis in human kidney and pancreas tissues. Corresponding DBT RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-89522 (A) shows similar protein distribution across tissues to independent antibody NBP1-85964 (B).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

UBE2E2 Antibody
NBP1-92556
Western Blot: UBE2E2 Antibody [NBP1-92556] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/Immunofluorescence: UBE2E2 Antibody [NBP1-92556] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

MASA Antibody
NBP2-13961
Western Blot: MASA Antibody [NBP2-13961] - Analysis in control (vector only transfected HEK293T lysate) and ENOPH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: MASA Antibody [NBP2-13961] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

TRIM25 Antibody
NBP2-20710
Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP2-20710] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: TRIM25 protein stained by TRIM25 antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
RIG-I Antibody - BSA Free
NBP2-27419
Western Blot: RIG-I Antibody [NBP2-27419] - Analysis of 293 lysate in the 1) absence and 2) presence of immunizing peptide using RIG-I antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: RIG-I Antibody [NBP2-27419] - Formalin-fixed, paraffin-embedded human breast stained with RIG-I antibody at 10 ug/ml.

Rabbit Polyclonal
Species Human, Primate
Applications WB, IHC, IHC-P

1 Publication
STAT1 [p Tyr701] Antibody [Unconjugated]
AF2894
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-a2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, CyTOF-ready

     1 Review

11 Publications
Jak1 Antibody (413104) [Unconjugated]
MAB4260
Jurkat human acute T cell leukemia cell line was stained with Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260, filled histogram) or isotype control antibody (Catalog # <a class=NoLineLink href='http://www.rndsystems.com/search?keywords=MAB0061'>MAB0061</a>, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')<sub>2</sub> Secondary Antibody (Catalog # <a class=NoLineLink href=Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

Rat Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     1 Review

2 Publications
ISG15/UCRP [7-Amino-4-Methyl Coumarin]
UL-553


Species Human
Applications EnzAct

12 Publications
Butyrylcholinesterase/BCHE Antibody [Unconjugated]
AF9024
Western blot shows lysates of mouse heart tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Polyclonal Antibody (Catalog # AF9024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Butyrylcholinesterase/BCHE was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9024) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Goat Polyclonal
Species Mouse, Rat
Applications WB, IHC

2 Publications
Elastase Antibody (CELA3B/1257) - Azide and BSA Free
NBP2-54301
Western Blot: Elastase Antibody (CELA3B/1257) - Azide and BSA Free [NBP2-54301] - Western Blot Analysis of PANC-1 cell lysate using Elastase antibody (CELA3B/1257).Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) - Azide and BSA Free [NBP2-54301] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

UbcH8/Ube2L6 Antibody (k1H3) - BSA Free
NBP1-04351
Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysateImmunocytochemistry/Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - ICC/IF analysis of UBE2L6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UBE2L6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

HERC5 Antibody
H00051191-A01
Western Blot: HERC5 Antibody [H00051191-A01] - Detection against Immunogen (38.21 KDa) .

Mouse Polyclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

7 Publications
IRF3 Antibody (SD2062)
NBP2-67741
Western Blot: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of IRF3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysateImmunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

2 Publications
IFN-alpha 1 Antibody
NBP2-75930
Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

USP18 Antibody
NB600-893
Western Blot: USP18 Antibody [NB600-893] - Analysis of a band 43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.Western blot using Affinity Purified anti-UBP43 antibody. Lane 1: HeLa nuclear extracts

Rabbit Polyclonal
Species Human
Applications WB, ELISA, ICC/IF

ILT6/CD85e/LILRA3 Antibody (2E9)
H00011026-M01
Western Blot: ILT6/CD85e/LILRA3 Antibody (2E9) [H00011026-M01] - Analysis of LILRA3 expression in transfected 293T cell line by LILRA3 monoclonal antibody (M01), clone 2E9.Lane 1: LILRA3 transfected lysate(50.485 KDa).Lane 2: Non-transfected lysate.

Mouse Monoclonal
Species Human
Applications WB, ELISA

6 Publications