VAMP-1/VAMP-2 Antibody (SP10) Summary
Immunogen |
Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies. |
Marker |
Synaptic Vesicles Marker |
Specificity |
VAMP2 (SP10) |
Isotype |
IgM |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
VAMP1 |
Purity |
Ammonium sulfate precipitation |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
- Western Blot 1:4000
|
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.2), 0.1mM PMSF and 50% Glycerol |
Preservative |
No Preservative |
Concentration |
1.0 mg/ml |
Purity |
Ammonium sulfate precipitation |
Alternate Names for VAMP-1/VAMP-2 Antibody (SP10)
Background
VAMP2 (Synaptobrevin 2) is a small integral membrane protein found specifically in synaptic vesicles in neurons. Synaptobrevins/VAMPs, syntaxins, and the 25 kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP2 is one of a family of integral membrane proteins of 11-13 kDa with the N terminal region exposed to the cytoplasm and a C terminal transmembrane domain. Two isoforms identified in the mammalian CNS Synaptobrevin 1/VAMP 1 and Synaptobrevin 2/VAMP 2 differ in their distribution within different brain regions. Synaptobrevin/VAMP is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2/VAMP 2 are present in many other tissues where the protein resides on specialized microvesicles. In non-neuronal cells, the third isoform, Cellubrevin/VAMP 3, is present where it is localized to an endosomal membrane pool. Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C terminal portion of the molecule.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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