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Syntaxin 1A Antibody (2B2F10)

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Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ...read more
Immunohistochemistry: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunofluorescence analysis of mouse brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of various cell lines, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit ...read more
Immunohistochemistry-Paraffin: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunohistochemistry of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens).Perform ...read more
Immunohistochemistry: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunofluorescence analysis of rat brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
2B2F10
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Syntaxin 1A Antibody (2B2F10) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Syntaxin 1A (Q16623). MKDRTQELRTAKDSDDDDDVAVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSI
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
STX1A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Syntaxin 1A Antibody (2B2F10)

  • Brain Syntaxin
  • HPC-1
  • Neuron-specific antigen HPC-1
  • p35-1
  • STX1A
  • STX1P35-1
  • SYN1A
  • syntaxin 1A (brain)
  • Syntaxin 1A
  • syntaxin-1A

Background

Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol STX1A