Tryptophan hydroxylase 2 Antibody - BSA Free

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 8 applications: B/N, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, WB.

Showing Publications 1 - 10 of 53. Show All 53 Publications. Collapse Publications.

Publications using NB100-74555	Applications	Species
Higuchi Y, Tada T, Nakachi T, Arakawa H Serotonergic circuit dysregulation underlying autism-related phenotypes in BTBR mouse model of autism Neuropharmacology 2023-06-09 [PMID: 37301467] (IHC-Fr, Mouse) Details: 1:800 dilution	IHC-Fr	Mouse
Wang J, Mei Y, Zhang X et al. Aberrant serotonergic signaling contributes to the hyperexcitability of CA1 pyramidal neurons in a mouse model of Alzheimer's disease Cell reports 2023-02-22 [PMID: 36821438] (IHC-FrFl, Mouse) Details: Dilution used in IHC-FrFl 1:500	IHC-FrFl	Mouse
Ganley RP, de Sousa MM, Werder K et al. Targeted anatomical and functional identification of antinociceptive and pronociceptive serotonergic neurons that project to the spinal dorsal horn eLife 2023-02-08 [PMID: 36752606] (IHC, Mouse)	IHC	Mouse
Jansch C Effects of SLC2A3 copy number variants on neurodevelopment and glucose metabolism in ADHD patient-specific neurons Thesis 2021-01-01 (ICC/IF, Human)	ICC/IF	Human
Liu S, Xiang K, Yuan F, Xiang M Generation of self-organized autonomic ganglion organoids from fibroblasts iScience 2023-03-01 [PMID: 36922996]
Pinto DFC Convergent Dopamine and Serotonin Reward Signals Govern Associative Learning Thesis 2023-01-01
Li W, Shen Z, Yin X et al. Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors Translational psychiatry 2023-11-22 [PMID: 37993435] (IHC, Mouse) Details: 1:500 dilution	IHC	Mouse
Cheng H, Lou Q, Lai N et al. Projection-defined median raphe Pet+ subpopulations are diversely implicated in seizure Neurobiology of disease 2023-11-15 [PMID: 37977434] (IHC, Mouse) Details: 1:1000 dilution, Free floating sections	IHC	Mouse
Marcinkiewcz C, Wang R, Khan K et al. Alcohol inhibits sociability via serotonin inputs to the nucleus accumbens Research Square 2023-08-18 [PMID: 37461716]
Furukawa M, Tada H, Raju R et al. Long-Term Soft-Food Rearing in Young Mice Alters Brain Function and Mood-Related Behavior Nutrients 2023-05-20 [PMID: 37242280] (IHC, Mouse)	IHC	Mouse
Lim J, Bang Y, Kim KM, Choi HJ. Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors Frontiers in Pharmacology 2023-01-10 [PMID: 36703746] (B/N)	B/N
Pomrenze MB, Cardozo Pinto DF, Neumann PA et al. Modulation of 5-HT release by dynorphin mediates social deficits during opioid withdrawal Neuron 2022-09-29 [PMID: 36202097]
Luo N, Mosialou I, Capulli M et al. A neuronal action of sirtuin 1 suppresses bone mass in young and aging mice The Journal of clinical investigation 2022-10-04 [PMID: 36194488] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Baytas O, Kauer J, Morrow E Loss of mitochondrial enzyme GPT2 causes early neurodegeneration in locus coeruleus Neurobiology of Disease 2022-07-01 [PMID: 35908744] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Beier K. Modified viral-genetic mapping reveals local and global connectivity relationships of ventral tegmental area dopamine cells eLife 2022-05-23 [PMID: 35604019]
Pouchelon G, Dwivedi D, Bollmann Y Et al. The organization and development of cortical interneuron presynaptic circuits are area specific Cell reports 2021-11-09 [PMID: 34758329] (IF/IHC, Mouse)	IF/IHC	Mouse
Lee JS, Lee SB, Kim DW Et al. Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation Science advances 2021-11-05 [PMID: 34739315] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ortuno MJ, Schneeberger M, Ilanges A Et al. Melanocortin 4 receptor stimulation prevents anti-depressant-associated weight gain in mice caused by long-term fluoxetine exposure The Journal of clinical investigation 2021-10-21 [PMID: 34673574] (IF/IHC, Mouse)	IF/IHC	Mouse
Kang JY, Kim DY, Lee JS et al. Korean Red Ginseng Ameliorates Fatigue via Modulation of 5-HT and Corticosterone in a Sleep-Deprived Mouse Model Nutrients 2021-09-06 [PMID: 34578998] (ICC/IF)	ICC/IF
Seigneur E, Wang J, Dai J et al. Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors Molecular psychiatry 2021-06-22 [PMID: 34158618]
Vieira JS, de Souza GR, Kalil-Cutti B et al. Post-traumatic stress disorder increases pain sensitivity by reducing descending noradrenergic and serotoninergic modulation Behavioural brain research 2021-05-14 [PMID: 34000338]
Cheng H, Qi Y, Lai N et al. Inhibition of hyperactivity of the dorsal raphe 5-HTergic neurons ameliorates hippocampal seizure CNS neuroscience & therapeutics 2021-05-06 [PMID: 33955651] (IF/IHC)	IF/IHC
Huerta-Ocampo I, Dautan D, Gut N et al. Whole-brain mapping of monosynaptic inputs to midbrain cholinergic neurons Sci Rep 2021-04-27 [PMID: 33907215]
Konig N, Bimpisidis Z, Dumas S, Wallen-Mackenzie A Selective Knockout of the Vesicular Monoamine Transporter 2 (Vmat2) Gene in Calbindin2/Calretinin-Positive Neurons Results in Profound Changes in Behavior and Response to Drugs of Abuse Sci Transl Med 2020-11-25 [PMID: 33240055]
Bell BJ, Wang AA, Kim DW et al. Characterization of mWake expression in the murine brain Journal of Comparative Neurology 2021-06-01 [PMID: 33140455]
Okaty BW, Sturrock N, Escobedo Lozoya Y et al. A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons Elife 2020-06-22 [PMID: 32568072] (ICC/IF, Mouse)	ICC/IF	Mouse
Tudeau L, AcuNa MA, Albisetti GW et al. Mice lacking spinal alpha 2GABAA receptors: Altered GABAergic neurotransmission, diminished GABAergic antihyperalgesia, and potential compensatory mechanisms preventing a hyperalgesic phenotype Brain Res. 2020-05-18 [PMID: 32439345] (IF/IHC, Mouse)	IF/IHC	Mouse
Dombret C, NaulE L, Trouillet AC et al. Effects of neural estrogen receptor beta deletion on social and mood-related behaviors and underlying mechanisms in male mice Sci Rep 2020-04-10 [PMID: 32277160] (IF/IHC, Mouse)	IF/IHC	Mouse
Lee JS, Jeon YJ, Park SY, Son CG An Adrenalectomy Mouse Model Reflecting Clinical Features for Chronic Fatigue Syndrome Biomolecules 2020-01-01 [PMID: 31906307] (Mouse)		Mouse
Ren J, Isakova A, Friedmann D et al. Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei Elife 2019-10-24 [PMID: 31647409] (ICC/IF, Mouse)	ICC/IF	Mouse
Li Y, Li CY, Xi W et al. Rostral and Caudal Ventral Tegmental Area GABAergic Inputs to Different Dorsal Raphe Neurons Participate in Opioid Dependence Neuron 2019-02-20 [PMID: 30638902] (IF/IHC, Mouse)	IF/IHC	Mouse
Thier MC, Hommerding O, Panten J et al. Identification of Embryonic Neural Plate Border Stem Cells and Their Generation by Direct Reprogramming from Adult Human Blood Cells Cell Stem Cell 2018-12-12 [PMID: 30581079] (FLOW, Mouse)	FLOW	Mouse
Dosumu-Johnson RT, Cocoran AE, Chang Y et al. Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery. Elife. 2018-10-23 [PMID: 30350781] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Soiza-Reilly M, Meye FJ, Olusakin J et al. SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior. Mol. Psychiatry. 2018-10-02 [PMID: 30279456] (IF/IHC, Mouse)	IF/IHC	Mouse
Ehlinger DG, Commons KG. Cav1.2L-type calcium channels regulate stress coping behavior via serotonin neurons. Neuropharmacology 2018-08-31 [PMID: 30176250] (ICC/IF, Mouse)	ICC/IF	Mouse
Ren J, Friedmann D, Xiong J et al. Anatomically Defined and Functionally Distinct Dorsal Raphe Serotonin Sub-systems Cell 2018-08-18 [PMID: 30146164] (IF/IHC, Mouse)	IF/IHC	Mouse
Ma J, Wang F, Yang J et al. Xiaochaihutang attenuates depressive/anxiety-like behaviors of social isolation-reared mice by regulating monoaminergic system, neurogenesis and BDNF expression Journal of Ethnopharmacology 2017-08-17 [PMID: 28687505] (Mouse)		Mouse
Natarajan R, Forrester L, Chiaia N, Yamamoto BK. Chronic Stress-induced Behavioral Changes Associated with Subregion Selective Serotonin Cell Death in the Dorsal Raphe. J. Neurosci. 2017-05-25 [PMID: 28546314] (ICC/IF, Rat)	ICC/IF	Rat
Pomeranz LE, Ekstrand MI, Latcha KN et al. Gene expression profiling with Cre-conditional pseudorabies virus reveals a subset of midbrain neurons that participate in reward circuitry. J. Neurosci. 2017-03-10 [PMID: 28283558] (IF/IHC, Mouse)	IF/IHC	Mouse
Goto S, Ogi H, Fushiki S, Itoh K. Prenatal and lactational bisphenol A exposure does not alter serotonergic neurons morphologically in the murine dorsal raphe nucleus. Brain Dev. 2017-02-21 [PMID: 28233694]
Coyle D, Murphy JM, Doyle B et al. Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis. World J Gastroenterol 2016-05-21 [PMID: 27217698] (WB)	WB
Barrett KT, Dosumu-Johnson RT, Daubenspeck JA et al. Partial Raphe Dysfunction in Neurotransmission Is Sufficient to Increase Mortality after Anoxic Exposures in Mice at a Critical Period in Postnatal Development. J. Neurosci. 2016-04-06 [PMID: 27053202] (ICC/IF, Mouse)	ICC/IF	Mouse
Commons KG. Ascending serotonin neuron diversity under two umbrellas. Brain Struct Funct. 2016-01-06 [PMID: 26740230] (IF/IHC, Mouse)	IF/IHC	Mouse
Alter SP, Stout KA, Lohr KM et al. Reduced vesicular monoamine transport disrupts serotonin signaling but does not cause serotonergic degeneration. Exp Neurol 2016-01-01 [PMID: 26428905] (IF/IHC)	IF/IHC
Naslund J, Studer E, Petterson R et al. Differences in anxiety-like behaviour within a batch of Wistar rats are associated with differences in serotonergic transmission, enhanced by acute SSRI administration and abolished by serotonin depletion Int. J. Neuropsychopharmacol. 2015-02-25 [PMID: 25716782] (WB, Rat)	WB	Rat
Stansley BJ, Yamamoto BK. Chronic L-Dopa Decreases Serotonin Neurons in a Sub-region of the Dorsal Raphe Nucleus. J Pharmacol. Exp. Ther. 2014-09-11 [PMID: 25212217]
Ray RS, Corcoran AE, Brust RD et al. Egr2-neurons control the adult respiratory response to hypercapnia Brain Res 2012-12-19 [PMID: 23261662] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Zhou W, Chen L, Paul J et al. The effects of glycogen synthase kinase-3beta in serotonin neurons PLoS One 2012-01-01 [PMID: 22912839] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Fukada M, Hanai A, Nakayama A, Suzuki T, Miyata N, Rodriguiz RM, Wetsel WC, Yao TP, Kawaguchi Y. Loss of deacetylation activity of hdac6 affects emotional behavior in mice. PLoS One;7(2):e30924. 2012-01-01 [PMID: 22328923]
Bang SJ, Jensen P, Dymecki SM, Commons KG. Projections and interconnections of genetically defined serotonin neurons in mice Eur J Neurosci 2012-01-01 [PMID: 22151329] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Soiza-Reilly M, Commons KG. Glutamatergic drive of the dorsal raphe nucleus. J Chem Neuroanat 41(4):247-55. doi: 10.1016/j.jchemneu.2011.04.004. 2011-07-01 [PMID: 21550397]
Tokuyama Y, Ingram SL, Woodward JS et al. Functional characterization of rhesus embryonic stem cell-derived serotonin neurons. Exp Biol Med (Maywood). 2010-05-01 [PMID: 20463306] (ICC/IF, Primate)	ICC/IF	Primate
Sullivan EL, Grayson B, Takahashi D et al. Chronic consumption of a high-fat diet during pregnancy causes perturbations in the serotonergic system and increased anxiety-like behavior in nonhuman primate offspring. J Neurosci 2010-03-10 [PMID: 20220017] (ICC/IF, IF/IHC, Primate)	ICC/IF, IF/IHC	Primate
Show All 53 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Luiz Alexandre Magno	IHC-Fr	Mouse	07/19/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested midbrain Species Mouse Lot 1068-102 Comments Comments This is a fantastic antibody to detect TPH2 in free floating slices from PFA-perfused mice. I use it with Alexa Fluor 488 2nd antibody.
	5 reviewed by: Verified Customer	ICC	Mouse	12/27/2011	View Summary Application Immunocytochemistry Sample Tested Mouse Species Mouse
Enlarge	4 reviewed by: Verified Customer	IHC-P	Mouse	10/25/2010	View Summary Application Immunohistochemistry-Paraffin Sample Tested Brain Species Mouse Lot K1137602

Array NB100-74555

• Tryptophan hydroxylase 2 Antibodies
• Tryptophan hydroxylase 2 Proteins