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![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunohistochemistry-Paraffin-NB100-74555-img0009.jpg "Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.")
![Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tryptophan Hydroxylase 2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NB100-74555-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan hydroxylase 2-positive double stained cells in the dorsal raphe nuclei. Representative photomicrographs were taken at magnifications of 100x. Quantification of 5-HT and Tryptophan hydroxylase 2. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2218-273X/10/1/71), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74555-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74555-img0006.jpg)
![Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot LUT. Image from verified customer review.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunohistochemistry-Free-Floating-NB100-74555-img0008.jpg)
![Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunoprecipitation-NB100-74555-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2447-img0003.jpg "Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Staining of paraffin embedded Human Brain Stem. Antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunohistochemistry-Paraffin-NB100-2447-img0001.jpg)
![Flow Cytometry: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Flow-Cytometry-NB100-2447-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2447-img0002.jpg)
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0016.jpg "Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.")
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the basal forebrain.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0011.jpg)
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the cerebral cortex.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0012.jpg)
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in serotonin neurons.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0013.jpg)
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human cerebral cortex shows moderate positivity in serotonergic neural fibers.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0014.jpg)
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-2990-Immunohistochemistry-Paraffin-NBP2-46646-img0015.jpg)
![Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77559] - Analysis of TPH2 in human stomach lysate.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NBP1-77559-img0001.jpg "Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77559] - Analysis of TPH2 in human stomach lysate.")
![Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77556] - Analysis of TPHZ in human stomach lysate.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NBP1-77556-img0001.jpg "Western Blot: Tryptophan hydroxylase 2 Antibody [NBP1-77556] - Analysis of TPHZ in human stomach lysate.")
![Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunofluorescence analysis of frozen mouse brain (raphe nucleus) tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33037, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-2682024159393.jpg "Immunohistochemistry-Frozen: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunofluorescence analysis of frozen mouse brain (raphe nucleus) tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33037, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Western blot analysis of Tryptophan hydroxylase 2 on different lysates with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/1,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 18 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33037) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-28820241249341.jpg)
![Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (PSH04-89) [NBP3-33037] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tryptophan hydroxylase 2 antibody (NBP3-33037) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33037) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33037_rabbit-tryptophan-hydroxylase-2-mab-psh04-89-29820241492660.jpg)
![Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-61104] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) using this antibody at 1:1000.](http://images.novusbio.com/fullsize/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NB100-61104-img0003.jpg "Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-61104] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) using this antibody at 1:1000.")