SMC5 Antibody Summary
Immunogen |
Recombinant protein encompassing a sequence within the center region of human SMC5. The exact sequence is proprietary. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
SMC5 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
- Western Blot 1:500-1:3000
|
Theoretical MW |
129 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%).
Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer |
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
Preservative |
0.01% Thimerosal |
Purity |
Antigen Affinity-purified |
Alternate Names for SMC5 Antibody
Background
Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for SMC5 Antibody (NBP2-20419). (Showing 1 - 1 of 1 FAQ).
-
What dilution should be used for immunoflourescence?
- Unfortunately, our product NBP2-20419 has only been validated in western blot, so we do not have a recommended dilution for IF. If you would like to try this product in IF with cells or tissue, you would qualify for our Innovator's Reward Program. You would just need to submit your results (positive or negative) as a review on the product and we will send you a credit for the purchase price of the antibody. You can find details of this program here: Innovators Reward Program
Secondary Antibodies
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Isotype Controls
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