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MCM10 Antibody [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 669

Order Details

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MCM10 Antibody [Janelia Fluor® 669] Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human Minichromosome Maintenance 10 using the numbering given in entry NP_060988.3 (GeneID 55388).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MCM10 Antibody [Janelia Fluor® 669]

  • CNA43homolog of yeast MCM10
  • DNA43
  • hsMCM10
  • MCM10 minichromosome maintenance deficient 10 (S. cerevisiae)
  • MCM10 minichromosome maintenance deficient 10
  • MGC126776
  • minichromosome maintenance complex component 10
  • PRO2249
  • protein MCM10 homolog

Background

MCM10 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol MCM10