![Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of Mouse spleen, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Western-Blot-NBP3-15386-img0006.jpg "Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of Mouse spleen, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | 9Z5W4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MCM3 (P25205). MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYV |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MCM3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 91 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MCM3 |
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of NIH-3T3 cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunocytochemistry-Immunofluorescence-NBP3-15386-img0004.jpg "Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of NIH-3T3 cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded human liver cancer using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunohistochemistry-Paraffin-NBP3-15386-img0002.jpg "Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded human liver cancer using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of various cell lines, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Western-Blot-NBP3-15386-img0005.jpg "Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of various cell lines, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of HeLa cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunocytochemistry-Immunofluorescence-NBP3-15386-img0003.jpg "Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of HeLa cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded rat spleen using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunohistochemistry-Paraffin-NBP3-15386-img0001.jpg "Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded rat spleen using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunoprecipitation: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM3 antibody (NBP3-15386). Western blot was performed from the immunoprecipitate using MCM3 antibody (NBP3-15386) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunoprecipitation-NBP3-15386-img0007.jpg "Immunoprecipitation: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM3 antibody (NBP3-15386). Western blot was performed from the immunoprecipitate using MCM3 antibody (NBP3-15386) at a dilition of 1:1000.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")