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CDC7 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CDC7 Antibody [NBP2-32708] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytokinetic bridge & mitotic spindle. Antibody stainng is shown in green.
Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human stomach, upper shows moderate nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CDC7 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREY
Predicted Species
Mouse (95%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CDC7 Protein (NBP2-32708PEP)
Reviewed Applications
Read 1 Review rated 2
using
NBP2-32708 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CDC7 Antibody

  • CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
  • CDC7 cell division cycle 7 (S. cerevisiae)
  • CDC7
  • CDC7L1
  • CDC7L1MGC117361
  • CDC7-related kinase
  • cell division cycle 7 (S. cerevisiae)
  • cell division cycle 7 homolog (S. cerevisiae)
  • cell division cycle 7-like protein 1
  • cell division cycle 7-related protein kinase
  • EC 2.7.11.1
  • HsCdc7
  • Hsk1
  • huCdc7
  • MGC126237
  • MGC126238

Background

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC7 Antibody (NBP2-32708) (0)

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Review for CDC7 Antibody (NBP2-32708) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: HCT116 cells.

Reviews using NBP2-32708:
Filter by Applications
Simple Western
(1)
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Filter by Species
HCT116 cells
(1)
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Images Ratings Applications Species Date Details
Simple Western CDC7 NBP2-32708
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2
reviewed by:
Verified Customer
Simple Western HCT116 cells 08/27/2020
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Summary

ApplicationSimple Western
Sample TestedHCT116 whole cell lysate,whole cell lysate
SpeciesHCT116 cells
LotR33001

Comments

Comments10 fold primary Ab dilution; 0.8mg/ml lysate conc
*Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for CDC7 Antibody (NBP2-32708) (0)

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mFluor Violet Conjugated Antibodies

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Verified Customer
08/27/2020
Application: Simple Western
Species: HCT116 cells

Bioinformatics

Gene Symbol CDC7