![Western Blot: MCM10 Antibody [NB100-253] - No relocalization or post-translational modifications of some components of the replication machinery after UVA radiation. Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. Subcellular localization of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, Mcm10, Orc2, PCNA, RPA32 in asynchronous cells (Asyncro.) or cells synchronized in early S-phase (Synchro.). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MCM10-Antibody-Western-Blot-NB100-253-img0003.jpg "Western Blot: MCM10 Antibody [NB100-253] - No relocalization or post-translational modifications of some components of the replication machinery after UVA radiation. Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. Subcellular localization of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, Mcm10, Orc2, PCNA, RPA32 in asynchronous cells (Asyncro.) or cells synchronized in early S-phase (Synchro.). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human Minichromosome Maintenance 10 using the numbering given in entry NP_060988.3 (GeneID 55388). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MCM10 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | By Western blot, this antibody recognizes bands around 112 kDa representing MCM10. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: MCM10 Antibody [NB100-253] - Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody NB100-253 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MCM10-Antibody-Western-Blot-NB100-253-img0002.jpg "Western Blot: MCM10 Antibody [NB100-253] - Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody NB100-253 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MCM10 Antibody [NB100-253] - Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. The fractions of chromatin-bound proteins and total soluble proteins were recovered at various time points post UVA. The expression levels of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, p-Mcm2(Ser40/41), Mcm10, Orc2, PCNA, and RPA32 were detected by Western blotting in denaturing and reducing conditions. Lamin A/C and GAPDH were used as loading control for the chromatin-bound and soluble fractions, respectively. The apparent molecular weight of each protein is indicated on the right side of each blot (panel 7A). The stars (*) indicate the position of the non specific bands detected by Orc2 antibody. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MCM10-Antibody-Western-Blot-NB100-253-img0004.jpg "Western Blot: MCM10 Antibody [NB100-253] - Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. The fractions of chromatin-bound proteins and total soluble proteins were recovered at various time points post UVA. The expression levels of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, p-Mcm2(Ser40/41), Mcm10, Orc2, PCNA, and RPA32 were detected by Western blotting in denaturing and reducing conditions. Lamin A/C and GAPDH were used as loading control for the chromatin-bound and soluble fractions, respectively. The apparent molecular weight of each protein is indicated on the right side of each blot (panel 7A). The stars (*) indicate the position of the non specific bands detected by Orc2 antibody. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY license.")
![Immunoprecipitation: MCM10 Antibody [NB100-253] - HeLa cell lysate (1 mg for IP). Similar results were obtained with HCT116 cells (notshown). Antibody: Affinity purified rabbit anti- NB100-253 used at 1 ug/mg lysate for IP and 0.5 ug/ml for WB. Detection: Chemiluminescence.](http://images.novusbio.com/fullsize/MCM10-Antibody-Immunoprecipitation-NB100-253-img0001.jpg "Immunoprecipitation: MCM10 Antibody [NB100-253] - HeLa cell lysate (1 mg for IP). Similar results were obtained with HCT116 cells (notshown). Antibody: Affinity purified rabbit anti- NB100-253 used at 1 ug/mg lysate for IP and 0.5 ug/ml for WB. Detection: Chemiluminescence.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")