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PDX-1/IPF1 Antibody [Unconjugated]

 (70 Publications)
 
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Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX-1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) ...read more
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of ...read more
PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
PDX-1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL ...read more
Differentiation of MODY3-iPSCs into pancreatic beta cells.(A) scheme of 6 step differentiation protocol (B), expression pattern of HNF1A mRNA during differentiation process, (C) Phase-contrast image of spheroids of ...read more
Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for PDX-1 mRNA (ACD RNAScope Probe, catalog #437088; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, Dual ISH-IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2419
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2419-SP
100% Guarantee on every product

PDX-1/IPF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Specificity
Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDX1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Unconjugated]

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDX-1/IPF1 Antibody (AF2419)(70)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Human`, Xenograft.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


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Flow Cytometry
(13)
ICC
(26)
IHC
(7)
IHC-Fr
(1)
IHC-P
(2)
Immunoprecipitation
(1)
Western Blot
(1)
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Human
(38)
Mouse
(2)
Rat
(1)
Human`
(1)
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(1)
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Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using AF2419 Applications Species
Lyu, X;Rowley, MJ;Kulik, MJ;Dalton, S;Corces, VG; Regulation of CTCF loop formation during pancreatic cell differentiation Nature communications 2023-10-09 [PMID: 37813869] (ICC, Human) ICC Human
Samuel D. Pollock, Israeli M. Galicia-Silva, Mai Liu, Zoe L. Gruskin, Juan R. Alvarez-Dominguez Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors STAR Protoc 2023-09-21 [PMID: 37738117]
Souralova, T;Hulinova, D;Jeseta, M;Ventruba, P;Hampl, A;Koutna, I; Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells Scientific reports 2023-09-12 [PMID: 37700192] (ICC, Human) ICC Human
Xisheng Li, Zhangjing Ma, Kathy O. Lui A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells STAR Protocols 2023-09-01 [PMID: 37392391]
Ito, R;Kimura, A;Hirose, Y;Hatano, Y;Mima, A;Mae, SI;Keidai, Y;Nakamura, T;Fujikura, J;Nishi, Y;Ohta, A;Toyoda, T;Inagaki, N;Osafune, K; Elucidation of HHEX in pancreatic endoderm differentiation using a human iPSC differentiation model Scientific reports 2023-05-29 [PMID: 37248264] (Flow Cytometry, ICC, Western Blot, Human) Flow Cytometry, ICC, Western Blot Human
FM Hermann, MF Kjærgaard, C Tian, U Tiemann, A Jackson, LR Olsen, M Kraft, PO Carlsson, IM Elfving, JLT Kettunen, T Tuomi, I Novak, H Semb An insulin hypersecretion phenotype precedes pancreatic beta cell failure in MODY3 patient-specific cells Cell Stem Cell, 2022-12-22;0(0):. 2022-12-22 [PMID: 36563694] (Flow Cytometry, ICC, IHC, Human, Xenograft) Flow Cytometry, ICC, IHC Human, Xenograft
Tereza Souralova, Daniela Rehakova, Michal Jeseta, Lenka Tesarova, Jindrich Beranek, Pavel Ventruba et al. The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy International Journal of Molecular Sciences 2022-10-18 [PMID: 36293356]
A Beucher, I Miguel-Esc, D Balboa, MG De Vas, MA Maestro, J Garcia-Hur, A Bernal, R Gonzalez-F, P Vargiu, H Heyn, P Ravassard, S Ortega, J Ferrer The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A Nature Cell Biology, 2022-10-06;0(0):. 2022-10-06 [PMID: 36202974] (IHC, Human) IHC Human
Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
EZ Sim, T Enomoto, N Shiraki, N Furuta, S Kashio, T Kambe, T Tsuyama, A Arakawa, H Ozawa, M Yokoyama, M Miura, S Kume Methionine metabolism regulates pluripotent stem cell pluripotency and differentiation through zinc mobilization Cell Reports, 2022-07-19;40(3):111120. 2022-07-19 [PMID: 35858556] (ICC, Human) ICC Human
Show All 70 Publications.

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Bioinformatics

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