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![HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with PGC1 alpha Antibody [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PGC1-alpha-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-04676-img0010.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0008.jpg "Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Western-Blot-NB300-537-img0006.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0009.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Western-Blot-NB300-537-img0002.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0004.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0005.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-Flow-Cytometry-NB300-537-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537] - PPAR alpha/NR1C1 was knocked down with siRNA in primary cultured rat cardiomyocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-3B6-PPAR-BSA-Free-Knockdown-Validated-NB300-537-img0010.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Western-Blot-NB600-636-img0015.jpg "Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 ug/mL, 1h at RT degrees . Secondary antibody: Anti-RABBIT IgG DyLight (R) 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunocytochemistry-Immunofluorescence-NB600-636-img0021.jpg)
![Immunohistochemistry: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing PPARalpha (h-i) immunolabeling. White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a-i = 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunohistochemistry-NB600-636-img0022.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Western-Blot-NB600-636-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ug/mL overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunocytochemistry-Immunofluorescence-NB600-636-img0012.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunohistochemistry-Paraffin-NB600-636-img0006.jpg)
![Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-Immunohistochemistry-Paraffin-NB600-636-img0007.jpg)
![ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody---N-terminal-ELISA-NB600-636-img0019.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.")
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/fullsize/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/fullsize/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NB120-19481-img0001.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.")
![Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Western-Blot-NB600-637-img0007.jpg "Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Immunohistochemistry of PPAR delta/NR1C2/NR1C2 antibody. Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta/NR1C2 in human myocardiocitys at 1:500.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Immunohistochemistry-NB600-637-img0008.jpg)
![ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - ELISA results of purified PPAR delta/NR1C2 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-ELISA-NB600-637-img0009.jpg)
![Immunohistochemistry of Rabbit Anti-PPAR delta (N terminal specific). Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta in human myocardiocitys at 1:500.](http://images.novusbio.com/fullsize/nb600-637_rabbit-polyclonal-ppar-delta-nr1c2-antibody-235202318143153.jpg)
![Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-Western-Blot-NBP2-22468-img0001.jpg "Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates and a molecular weight protein ladder.")
![Flow Cytometry: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of PPAR delta was done on jurkat cells. Cells were fixed with 70% ethanol for 10 minutes permeablized with 0.25% Triton (R) X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-Flow-Cytometry-NBP2-22468-img0003.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Western-Blot-NBP1-31834-img0010.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Chromatin Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31834-img0006.jpg)
![Western Blot: PPAR alpha Antibody [NBP1-31834] - Sample (30 ug of whole cell lysate) A: Rat primary hepatocyte 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PPAR-alpha-Antibody-Western-Blot-NBP1-31834-img0002.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Western-Blot-NBP1-31834-img0008.jpg)
![Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 ug of PPAR alpha antibody 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Immunoprecipitation-NBP1-31834-img0004.jpg)
![Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Western-Blot-NB120-3484-img0004.jpg "Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0003.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of PPAR alpha [pSer12] was done on MCF7 cells.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Flow-Cytometry-NB120-3484-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-[p-Ser12]-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3484-img0002.jpg)