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PPAR gamma/NR1C3 Antibody [HRP]

 (2 Publications)
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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22106AF350
Alexa Fluor 405
NBP2-22106AF405
Alexa Fluor 488
NBP2-22106AF488
Alexa Fluor 532
NBP2-22106AF532
Alexa Fluor 594
NBP2-22106AF594
Alexa Fluor 647
NBP2-22106AF647
Alexa Fluor 700
NBP2-22106AF700
Alexa Fluor 750
NBP2-22106AF750
Allophycocyanin
NBP2-22106APC
Biotin
NBP2-22106B
DyLight 350
NBP2-22106UV
DyLight 405
NBP2-22106V
DyLight 488
NBP2-22106G
DyLight 550
NBP2-22106R
DyLight 594
NBP2-22106DL594
DyLight 650
NBP2-22106C
DyLight 680
NBP2-22106FR
DyLight 755
NBP2-22106IR
FITC
NBP2-22106F
Janelia Fluor 549
NBP2-22106JF549
Janelia Fluor 646
NBP2-22106JF646
PE
NBP2-22106PE
PerCP
NBP2-22106PCP
Unconjugated
NBP2-22106
mFluor Violet 450 SE
NBP2-22106MFV450
mFluor Violet 500 SE
NBP2-22106MFV500
mFluor Violet 610 SE
NBP2-22106MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22106
BSA Free
NBP2-22106SS
BSA Free
NBP2-22106
100% Guarantee on every product

PPAR gamma/NR1C3 Antibody [HRP] Summary

Immunogen
A synthetic peptide made to an internal portion of the human PPAR gamma protein (between residues 20-120) [UniProt P37231]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System
Control
MFSD8 Overexpression Lysate
Publications
Read Publications using
NBP2-22106H in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27862692).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for PPAR gamma/NR1C3 Antibody [HRP]

  • CIMT1
  • NR1C3
  • NR1C3GLM1
  • Nuclear receptor subfamily 1 group C member 3
  • peroxisome proliferator-activated receptor gamma 1
  • peroxisome proliferator-activated receptor gamma
  • PPAR gamma
  • PPARG
  • PPARG1peroxisome proliferative activated receptor, gamma
  • PPARG2PPARgamma
  • PPAR-gamma

Background

Peroxisome proliferator-activated receptor gamma (PPARG), also known as nuclear receptor subfamily 1 group C member 3, NR1C3, GLM1, CIMT1, PPARG1, PPARG2, and PPARgamma, regulates adipocyte differentiation and binds peroxisome proliferators that include hypolipidemic drugs and fatty acids. PPARG regulates the peroxisomal beta-oxidation pathway of these fatty acids. PPARG activates transcription for acyl-CoA oxidase and regulates glucose homeostasis. PPARG is a member of the PPAR family, which is a subfamily of nuclear receptors. PPARG is found in the nucleus and the cytoplasm and is most prevalent in adipose tissue but also found in skeletal muscle, spleen, heart, liver, placenta, lung, and ovary. PPARG is found in three known isoforms (57.6, 54.8, and 21.6 kDa), which are produced by alternative splicing. The PPARG protein is subject to phosphorylation. PPARG has been linked to many diseases such as obesity, diabetes, atherosclerosis, and cancer. Recent research has indicated that mutations in PPARG play a role in non-alcoholic fatty liver disease (PMID: 22160635). Other research has found that using PPARG ligands as part of a chemotherapy treatment could help improve treatment for advanced pancreatic cancer (PMID: 22020928). Similar cancer research discovered that PPARG can be used to induce colorectal carcinoma cell death, making PPARG, in conjunction with survivin and caspase-3, a potential cancer cell regulator (PMID: 21765467).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPAR gamma/NR1C3 Antibody (NBP2-22106H)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-22106H Applications Species
Miller WR. PPARgamma agonism attenuates cocaine seeking behavior via association with ERk MAPk. Thesis. 1905-07-06 (WB, Rat) WB Rat
Miller WR, Fox RG, Stutz SJ et al. PPARgamma agonism attenuates cocaine cue reactivity Addict Biol. 2016-11-11 [PMID: 27862692]

Details:
Citation using the HRP form of this antibody.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

MFSD8 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.
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Bioinformatics

Gene Symbol PPARG