PPAR alpha/NR1C1 Antibody Summary
| Immunogen |
Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary. |
| Localization |
Nucleus |
| Predicted Species |
Porcine (92%), Rhesus Macaque (98%), Canine (92%). Backed by our 100% Guarantee. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
PPARA |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Chromatin Immunoprecipitation (ChIP)
- Immunohistochemistry-Paraffin
- Immunoprecipitation 1:100-1:500
- Western Blot 1:500-1:3000
|
| Theoretical MW |
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Queen Bee, Honeybee. Bovine (88%), Guinea pig (87%)
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer |
PBS (pH 7), 20% Glycerol |
| Preservative |
0.025% Proclin 300 |
| Purity |
Immunogen affinity purified |
Alternate Names for PPAR alpha/NR1C1 Antibody
Background
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for PPAR alpha/NR1C1 Antibody (NBP1-31834) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for PPAR alpha/NR1C1 Antibody (NBP1-31834). (Showing 1 - 1 of 1 FAQ).
-
Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
- Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
Secondary Antibodies
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Isotype Controls
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Blogs on PPAR alpha/NR1C1
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Western-Blot-NBP1-31834-img0010.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Chromatin Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-31834-img0006.jpg "Chromatin Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.")
![Western Blot: PPAR alpha Antibody [NBP1-31834] - Sample (30 ug of whole cell lysate) A: Rat primary hepatocyte 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PPAR-alpha-Antibody-Western-Blot-NBP1-31834-img0002.jpg "Western Blot: PPAR alpha Antibody [NBP1-31834] - Sample (30 ug of whole cell lysate) A: Rat primary hepatocyte 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Western-Blot-NBP1-31834-img0008.jpg "Western Blot: PPAR alpha/NR1C1 Antibody [NBP1-31834] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 ug of PPAR alpha antibody 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/PPAR-alpha-NR1C1-Antibody-Immunoprecipitation-NBP1-31834-img0004.jpg "Immunoprecipitation: PPAR alpha/NR1C1 Antibody [NBP1-31834] - IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 ug of PPAR alpha antibody 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")