![Western Blot: VGluT3 Antibody [NBP1-25973] - Analysis of tissue lysates prepared in different lysing buffers. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:1000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Western-Blot-NBP1-25973-img0002.jpg "Western Blot: VGluT3 Antibody [NBP1-25973] - Analysis of tissue lysates prepared in different lysing buffers. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:1000 incubated at 4C overnight.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | A synthetic peptide from mouse VGLuT3 conjugated to blue carrier protein was used as the antigen. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC17A8 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from whole antisera |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Unpurified |
| Reconstitution Instructions | Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
Secondary Antibodies |
Isotype Controls |
Research Areas for VGluT3 Antibody (NBP1-25973)Find related products by research area.
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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC17A8 |
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of rat brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0007.jpg "Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of rat brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0001.jpg "Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0003.jpg "Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0004.jpg "Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0005.jpg "Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of rat brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0006.jpg "Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of rat brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.")
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![Western Blot GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Western_Blot_19950.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")