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VGLUT1 Antibody (SR2075)

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Western Blot: VGLUT1 Antibody (SR2075) [NBP3-21883] - Western blot analysis using NBP3-21883 in Neuro-2a cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
SR2075
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VGLUT1 Antibody (SR2075) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human VGLUT1 (Uniprot #: Q9P2U7)
Specificity
Detects endogenous levels of total VGLUT1
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SLC17A7
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for VGLUT1 Antibody (SR2075)

  • BNPI
  • BNPIbrain-specific Na-dependent inorganic phosphate cotransporter
  • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
  • member 7
  • SLC17A7
  • solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
  • Solute carrier family 17 member 7
  • VGLUT1
  • VGLUT1vesicular glutamate transporter 1

Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC17A7