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SOX2 Antibody (245610) [Unconjugated]

Images

 
E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 ...read more
Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 ...read more
SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (MAB003, open histogram), ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more
SOX2 was detected in immersion fixed paraffin-embedded sections of glioblastoma using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 3 µg/mL for 1 hour at room temperature followed ...read more
Western blotting analysis of the different antigens studied in cell lines (NS and AC) and in primary tumors. (a) Stemness antigens and differentiation antigens prevail in NS and AC, respectively. (b) Densitometric ...read more
Double immunofluorescence staining of core and periphery in orthotopic model with five different patient derived GBM spheroid cultures.Tissues were stained with Dapi (blue), Vimentin (green) and Sox-2 (red), Bmi-1 (red) ...read more
Soluble factors increase the numbers of Hes3+ cells in the adult rat hippocampus.(a) Treatment with the soluble factors induced a powerful increase in the number of Sox2+ cells in the hilus [Quantifications are from ...read more
Hes3 is a marker of normal and cancer human stem cells.(A–C) Hes3+ cell in the striatum of non-cancerous adult human brain tissue (blood vessels identified by RECA-1 expression), human hemangioblastoma (HBM) biopsy ...read more
Double immunofluorescence staining of core and periphery of patient glioblastomas.Histological sections were stained with Dapi (blue), IDH1 (green) and Sox-2 (red) (AA-BE), Bmi-1 (red) (CA-DE) and Glut-3 (red) (EA to ...read more
Immunohistochemistry of a parallel section of Figure 1. (a) Poor GFAP expression. (b) High Nestin expression. (c) SOX2 is highly expressed in nuclei of the external slope of circumscribed necrosis. (d) REST expression, ...read more
Neural induction of hPSCs. (a) Both hPSC culture and neural differentiation were performed on LN521 substrate. Cortical neurons were induced with dual SMAD inhibition, expanded in the presence of FGF2, and matured with ...read more
Double immunofluorescence staining of core and periphery in orthotopic model with five different patient derived GBM spheroid cultures.Tissues were stained with Dapi (blue), Vimentin (green) and Sox-2 (red), Bmi-1 (red) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
Clone
245610
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

SOX2 Antibody (245610) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Specificity
Detects human, mouse, and rat SOX2 in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SOX2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 4 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 5 Reviews rated 4.4
using
MAB2018 in the following applications:

Publications
Read Publications using
MAB2018 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX2 Antibody (245610) [Unconjugated]

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF

Publications for SOX2 Antibody (MAB2018)(274)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Moose, Pan troglodytes (Chimpanzee), Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 10 applications: ChIP, ChIP-Seq, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(3)
ChIP-Seq
(1)
Flow Cytometry
(20)
ICC
(106)
IF
(1)
IHC
(34)
IHC-Fr
(10)
IHC-P
(10)
Immunoprecipitation
(1)
Western Blot
(28)
All Applications
Filter By Species
Human
(134)
Mouse
(44)
Rat
(7)
Moose
(1)
Pan troglodytes (Chimpanzee)
(1)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 274. Show All 274 Publications.
Publications using MAB2018 Applications Species
Ribeiro, M;Martins, S;Carvalho, T;Furtado, M;Sampaio Cabral, J;Brito, D;Carmo-Fonseca, M;da Rocha, ST; Generation of induced pluripotent stem cell lines from two unrelated individuals with familial hypertrophic cardiomyopathy carrying the MYBPC3 missense c.1484G>A mutation Stem cell research 2023-12-13 [PMID: 38104429] (ICC, Flow Cytometry, Human) ICC, Flow Cytometry Human
Hatim, O;Pavlinov, I;Xu, M;Linask, K;Beers, J;Liu, C;Baumgärtel, K;Gilbert, M;Spinner, N;Chen, C;Zou, J;Zheng, W; Generation of an Alagille syndrome (ALGS) patient-derived induced pluripotent stem cell line (TRNDi032-A) carrying a heterozygous mutation (p.Cys682Leufs*7) in the JAG1 gene Stem cell research 2023-10-18 [PMID: 37890331] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
Chen, Y;Kuang, J;Niu, Y;Zhu, H;Chen, X;So, KF;Xu, A;Shi, L; Multiple factors to assist human-derived induced pluripotent stem cells to efficiently differentiate into midbrain dopaminergic neurons Neural regeneration research 2024-04-01 [PMID: 37843228] (ICC, Human) ICC Human
Souralova, T;Hulinova, D;Jeseta, M;Ventruba, P;Hampl, A;Koutna, I; Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells Scientific reports 2023-09-12 [PMID: 37700192] (ICC, Human) ICC Human
Mann, JR;McKenna, ED;Mawrie, D;Papakis, V;Alessandrini, F;Anderson, EN;Mayers, R;Ball, HE;Kaspi, E;Lubinski, K;Baron, DM;Tellez, L;Landers, JE;Pandey, UB;Kiskinis, E; Loss of function of the ALS-associated NEK1 kinase disrupts microtubule homeostasis and nuclear import Science advances 2023-08-18 [PMID: 37585529] (ICC, Human) ICC Human
Funes, S;Gadd, DH;Mosqueda, M;Zhong, J;Jung, J;Shankaracharya, ;Unger, M;Cameron, D;Dawes, P;Keagle, PJ;McDonough, JA;Boopathy, S;Sena-Esteves, M;Lutz, C;Skarnes, WC;Lim, ET;Schafer, DP;Massi, F;Landers, JE;Bosco, DA; Expression of ALS-PFN1 impairs vesicular degradation in iPSC-derived microglia bioRxiv : the preprint server for biology 2023-06-01 [PMID: 37398081] (ICC, Human) ICC Human
Tam, KW;Wong, CY;Wu, KL;Lam, G;Liang, X;Wong, WT;Li, MT;Liu, WY;Cai, S;Shea, GK;Shum, DK;Chan, YS; IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries Cells 2023-05-25 [PMID: 37296600] (ICC, Human) ICC Human
Miho Isoda, Tsukasa Sanosaka, Ryo Tomooka, Yo Mabuchi, Munehisa Shinozaki, Tomoko Andoh-Noda et al. Mesenchymal properties of iPSC-derived neural progenitors that generate undesired grafts after transplantation Communications Biology 2023-06-07 [PMID: 37286713]
Robertson, FL;O'Duibhir, E;Gangoso, E;Bressan, RB;Bulstrode, H;Marqu�s-Torrej�n, M�;Ferguson, KM;Blin, C;Grant, V;Alfazema, N;Morrison, GM;Pollard, SM; Elevated FOXG1 in glioblastoma stem cells cooperates with Wnt/?-catenin to induce exit from quiescence Cell reports 2023-05-26 [PMID: 37243590] (Western Blot, Mouse) Western Blot Mouse
Jessica A Cooke, Andrew P Voigt, Michael A Collingwood, Nicholas E Stone, S Scott Whitmore, Adam P DeLuca et al. Propensity of Patient-Derived iPSCs for Retinal Differentiation: Implications for Autologous Cell Replacement Stem Cells Translational Medicine 2023-06-15 [PMID: 37221451]
Show All 274 Publications.

Reviews for SOX2 Antibody (MAB2018) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using MAB2018:
Filter by Applications
IF
(5)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Rat 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22791629
SpeciesRat
  5
reviewed by:
Verified Customer
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19824823
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Mouse 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19825428
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Other 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22573702
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX2 Antibody (MAB2018) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SOX2 Products

Blogs on SOX2. Showing 1-10 of 13 blog posts - Show all blog posts.

Spheroids vs. Organoids: Which 3D Cell Culture Model is Best for You?
By Jennifer Jones, M.S.Spheroids and organoids are two words that, like “butter” and “margarine”, are often referred to interchangeably but have distinct meanings. The progression and adopt...  Read full blog post.


  Read full blog post.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

SOX2: an Important Stem Cell Transcription Factor
SOX2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Sox-2 was found to be expressed by dividing neural progenitor cells. Constitutive expression of SOX2 has also been shown to ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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Recent Reviews

4.4
5
2
4
3
3
0
2
0
1
0

 
Verified Customer
02/16/2015
Application: IF
Species: Rat

 
Verified Customer
02/16/2015
Application: IF
Species: Human

 
Verified Customer
02/16/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol SOX2
Uniprot