![Western Blot: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis using ZFP42 mouse mAb against NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/Rex-1-ZFP42-Antibody-5E11A6-Western-Blot-NBP2-37357-img0004.jpg "Western Blot: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis using ZFP42 mouse mAb against NIH/3T3 cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, CyTOF-ready |
| Clone | 5E11A6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Rex-1/ZFP42 (AA: 249-310) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZFP42 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
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| Theoretical MW | 34.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Publication using NBP2-37357 | Applications | Species |
|---|---|---|
| Fu X, Wu X, Djekidel MN, Zhang Y Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic stem cells Nat. Cell Biol. 2019-06-17 [PMID: 31209294] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ZFP42 |
![Immunohistochemistry-Paraffin: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis of bladder cancer tissues using ZFP42 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Rex-1-ZFP42-Antibody-5E11A6-Immunohistochemistry-NBP2-37357-img0007.jpg "Immunohistochemistry-Paraffin: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis of bladder cancer tissues using ZFP42 mouse mAb with DAB staining.")
![Flow Cytometry: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis of HEK293 cells using ZFP42 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Rex-1-ZFP42-Antibody-5E11A6-Flow-Cytometry-NBP2-37357-img0005.jpg "Flow Cytometry: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis of HEK293 cells using ZFP42 mouse mAb (green) and negative control (red).")
![Western Blot: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis using ZFP42 mAb against human ZFP42 recombinant protein. (Expected MW is 32.7 kDa)](http://images.novusbio.com/fullsize/Rex-1-ZFP42-Antibody-5E11A6-Western-Blot-NBP2-37357-img0002.jpg "Western Blot: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis using ZFP42 mAb against human ZFP42 recombinant protein. (Expected MW is 32.7 kDa)")
![Western Blot: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis using ZFP42 mAb against HEK293 (1) and ZFP42 (AA: 249-310)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Rex-1-ZFP42-Antibody-5E11A6-Western-Blot-NBP2-37357-img0003.jpg "Western Blot: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis using ZFP42 mAb against HEK293 (1) and ZFP42 (AA: 249-310)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis of lung cancer tissues using ZFP42 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Rex-1-ZFP42-Antibody-5E11A6-Immunohistochemistry-NBP2-37357-img0006.jpg "Immunohistochemistry-Paraffin: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Analysis of lung cancer tissues using ZFP42 mouse mAb with DAB staining.")
![ELISA: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Rex-1-ZFP42-Antibody-5E11A6-ELISA-NBP2-37357-img0001.jpg "ELISA: Rex-1/ZFP42 Antibody (5E11A6) [NBP2-37357] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")