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OLFM-L3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: OLFM-L3 Antibody [NBP2-15055] - Analysis of methanol-fixed A549, using Olfactomedin-like 3 antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: OLFM-L3 Antibody [NBP2-15055] - Mouse heart. Olfactomedin-like 3 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: OLFM-L3 Antibody [NBP2-15055] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OLFML3 antibody [N1C1] diluted at 1:1000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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OLFM-L3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human OLFM-L3. The exact sequence is proprietary.
Predicted Species
Rat (93%), Rhesus Macaque (99%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OLFML3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for OLFM-L3 Antibody

  • HNOEL-iso
  • HNOEL-isoOLF44
  • hOLF44
  • OLF44
  • olfactomedin-like 3
  • olfactomedin-like protein 3
  • OLFML3
  • OLFM-L3

Background

Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol OLFML3
Uniprot