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CEACAM1/CD66a Antibody [Unconjugated]

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Western blot shows lysates of mouse liver tissue and mouse small intestine tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CEACAM-1/CD66a between approximately 110 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse liver tissue and mouse small intestine tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) ...read more
Mouse splenocytes were stained with Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ...read more
CEACAM‑1/CD66a was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) at 5 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6480-SP
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CEACAM1/CD66a Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-1/CD66a
Met1-Gly428
Accession # P31809
Specificity
Detects mouse CEACAM-1/CD66a in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Ceacam1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF6480 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM1/CD66a Antibody [Unconjugated]

  • antigen CD66
  • BGP1
  • BGP-1
  • BGP1BGPBiliary glycoprotein 1
  • BGPI
  • Biliary Glycoprotein 1
  • biliary glycoprotein adhesion molecule
  • carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
  • carcinoembryonic antigen-related cell adhesion molecule 1
  • CD66a antigen
  • CD66a
  • Cea-1
  • CEACAM1
  • CEACAM-1
  • Hv-1
  • Hv-2
  • MHVR

Background

CEACAM-1 (Carcinoembryonic antigen-related cell adhesion molecule 1; also BGP-1, CD66a and MHVR1) is a 110-120 kDa member of the CEACAM subfamily, CEA family of proteins. It has a wide expression pattern, being found on neutrophils, dendritic cells, endothelial cells, colonic epithelium and hepatocytes. It mediates cell adhesion, and appears to regulate insulin levels and signaling by interacting with the insulin receptor. It also demonstrates proangiogenic effects by inducing endothelial cells to proliferate and form capillary-like tubules. Finally, CEACAM-1 is a known receptor for mouse hepatitis virus. Mature mouse CEACAM-1 is a 487 amino acid (aa) type I transmembrane glycoprotein. Its contains a 394 aa extracellular region (aa 35-428) that shows one V-type (aa 35-142) and three C2-type
(aa 147-411) Ig-like domains, plus a 74 aa cytoplasmic domain. Three alternate splice forms exist. One contains a four aa substitution for aa 455-521, a second shows a Gln substitution for aa 142-322, and a third possesses a combination of the first two splice patterns. CEACAM-1 forms homodimers. Over aa 35-428, mouse CEACAM-1 shares 56% and 70% aa identity with human and rat CEACAM-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CEACAM1/CD66a
Antibody [Unconjugate
AF6480
CEACAM1/CD66a Antibody [Unconjugated]
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for CEACAM1/CD66a Antibody (AF6480)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


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IHC
(1)
IHC-Fr
(1)
Western Blot
(1)
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Mouse
(3)
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Showing Publications 1 - 5 of 5.
Publications using AF6480 Applications Species
SR Rosenbaum, M Tiago, S Caksa, C Capparelli, TJ Purwin, G Kumar, M Glasheen, D Pomante, D Kotas, I Chervoneva, AE Aplin SOX10 requirement for melanoma tumor growth is due, in part, to immune-mediated effects Cell Reports, 2021-12-07;37(10):110085. 2021-12-07 [PMID: 34879275] (Western Blot, Mouse) Western Blot Mouse
Malgorzata Ciurkiewicz, Stefan Floess, Michael Beckstette, Maren Kummerfeld, Wolfgang Baumgärtner, Jochen Huehn et al. Transcriptome analysis following neurotropic virus infection reveals faulty innate immunity and delayed antigen presentation in mice susceptible to virus‐induced demyelination Brain Pathology 2021-11-01 [PMID: 34231271]
Kojiro Nakamura, Shoichi Kageyama, Fady M. Kaldas, Hirofumi Hirao, Takahiro Ito, Kentaro Kadono et al. Hepatic CEACAM1 expression indicates donor liver quality and prevents early transplantation injury Journal of Clinical Investigation 2020-04-20 [PMID: 32027621]
IA Malik, G Malik, P Ströbel, J Wilting Development of A New Mouse Model for Intrahepatic Cholangiocellular Carcinoma: Accelerating Functions of Pecam-1 Cancers (Basel), 2019-07-24;11(8):. 2019-07-24 [PMID: 31344919] (IHC-Fr, Mouse) IHC-Fr Mouse
A Schmidt, TV Woedtke, J Stenzel, T Lindner, S Polei, B Vollmar, S Bekeschus One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet Int J Mol Sci, 2017-04-19;18(4):. 2017-04-19 [PMID: 28422070] (IHC, Mouse) IHC Mouse

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Bioinformatics

Gene Symbol Ceacam1
Uniprot