![Western Blot: NAPA Antibody [NBP1-32493] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha SNAP antibody [N2C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NAPA-Antibody-Western-Blot-NBP1-32493-img0010.jpg "Western Blot: NAPA Antibody [NBP1-32493] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha SNAP antibody [N2C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary. |
| Localization | Membrane, Peripheral membrane protein |
| Predicted Species | Porcine (97%), Bovine (98%), Rhesus Macaque (100%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NAPA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunoprecipitation Assay-dependent dilution |
| Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for NAPA Antibody (NBP1-32493)Find related products by research area.
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| Gene Symbol | NAPA |
![Immunocytochemistry/Immunofluorescence: NAPA Antibody [NBP1-32493] - DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha SNAP protein stained by alpha SNAP antibody [N2C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NAPA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32493-img0012.jpg "Immunocytochemistry/Immunofluorescence: NAPA Antibody [NBP1-32493] - DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha SNAP protein stained by alpha SNAP antibody [N2C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: NAPA Antibody [NBP1-32493] - Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] ) dilution: 1:500.](http://images.novusbio.com/fullsize/NAPA-Antibody-Immunohistochemistry-Paraffin-NBP1-32493-img0008.jpg "Immunohistochemistry-Paraffin: NAPA Antibody [NBP1-32493] - Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] ) dilution: 1:500.")
![Western Blot: NAPA Antibody [NBP1-32493] - NAPA Antibody Sample(30 ug whole cell lysate)A:Hep G2 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NAPA-Antibody-Western-Blot-NBP1-32493-img0007.jpg "Western Blot: NAPA Antibody [NBP1-32493] - NAPA Antibody Sample(30 ug whole cell lysate)A:Hep G2 10% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: NAPA Antibody [NBP1-32493] - NAPA Antibody Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NAPA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32493-img0005.jpg "Immunocytochemistry/Immunofluorescence: NAPA Antibody [NBP1-32493] - NAPA Antibody Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")