![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0014.jpg "Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the amino acids: LGVKNSEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAPRRTEITIVKPQESA |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SEPT9 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Septin-9 Antibody (NBP2-13294)Find related products by research area.
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| Gene Symbol | SEPT9 |
![Western Blot: Septin-9 Antibody [NBP2-13294] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using anti-SEPT9 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP2-13294-img0008.jpg "Western Blot: Septin-9 Antibody [NBP2-13294] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using anti-SEPT9 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP2-13294] - Staining of human cell line A-431 shows localization to actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13294-img0006.jpg "Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP2-13294] - Staining of human cell line A-431 shows localization to actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0015.jpg "Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0009.jpg "Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human skeletal muscle shows no positivity in myocytes.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0010.jpg "Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0011.jpg "Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0013.jpg "Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")