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Recombinant Human Nanog His Protein

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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

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Recombinant Human Nanog His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-154 of Human Nanog

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NANOG
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Nanog His Protein

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed. Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol NANOG