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Nanog Antibody (5A10) [DyLight 680]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
5A10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-04320AF350
Alexa Fluor 405
NBP1-04320AF405
Alexa Fluor 488
NBP1-04320AF488
Alexa Fluor 532
NBP1-04320AF532
Alexa Fluor 594
NBP1-04320AF594
Alexa Fluor 647
NBP1-04320AF647
Alexa Fluor 700
NBP1-04320AF700
Alexa Fluor 750
NBP1-04320AF750
Biotin
NBP1-04320B
DyLight 350
NBP1-04320UV
DyLight 405
NBP1-04320V
DyLight 488
NBP1-04320G
DyLight 550
NBP1-04320R
DyLight 594
NBP1-04320DL594
DyLight 650
NBP1-04320C
DyLight 755
NBP1-04320IR
FITC
NBP1-04320F
HRP
NBP1-04320H
Janelia Fluor 549
NBP1-04320JF549
Janelia Fluor 646
NBP1-04320JF646
Unconjugated
NBP1-04320
mFluor Violet 450 SE
NBP1-04320MFV450
mFluor Violet 500 SE
NBP1-04320MFV500
mFluor Violet 610 SE
NBP1-04320MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-04320
BSA Free
NBP1-04320
BSA Free
NBP1-04320SS
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Nanog Antibody (5A10) [DyLight 680] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Recombinant human Nanog (1-154aa) purified from E. coli (NP_079141).
Marker
Embryonic Stem Cell Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NANOG
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Nanog Antibody (5A10) [DyLight 680]

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Nanog Antibody
(5A10) [DyLight 680]
NBP1-04320FR
Nanog Antibody (5A10) [DyLight 680]
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.
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Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot