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Musashi-1 Antibody (OTI1G8)

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Western Blot: Musashi 1 Antibody (1G8) [NBP2-03434] Analysis of extracts (35ug) from 9 different cell lines by using anti-Musashi 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: Musashi 1 Antibody (1G8) [NBP2-03434] Analysis of extracts (35ug) from 9 different cell lines by using anti-Musashi 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Musashi 1.
Immunohistochemistry-Paraffin: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of paraffin-embedded Human pancreas tissue using anti-Musashi 1 mouse monoclonal antibody.
Flow Cytometry: Musashi 1 Antibody (1G8) [NBP2-03434] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Musashi 1 antibody, and then ...read more
Western Blot: Musashi 1 Antibody (1G8) [NBP2-03434] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Musashi 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Musashi 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Musashi 1 mouse monoclonal antibody.
Flow Cytometry: Musashi 1 Antibody (1G8) [NBP2-03434] - Analysis of Hela cells, using anti-Musashi 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72828AF350
Alexa Fluor 405
NBP2-72828AF405
Alexa Fluor 488
NBP2-72828AF488
Alexa Fluor 532
NBP2-72828AF532
Alexa Fluor 594
NBP2-72828AF594
Alexa Fluor 647
NBP2-72828AF647
Alexa Fluor 700
NBP2-72828AF700
Alexa Fluor 750
NBP2-72828AF750
Allophycocyanin
NBP2-72828APC
Biotin
NBP2-72828B
DyLight 350
NBP2-72828UV
DyLight 405
NBP2-72828V
DyLight 488
NBP2-72828G
DyLight 550
NBP2-72828R
DyLight 594
NBP2-72828DL594
DyLight 650
NBP2-72828C
DyLight 680
NBP2-72828FR
DyLight 755
NBP2-72828IR
FITC
NBP2-72828F
HRP
NBP2-72828H
Janelia Fluor 549
NBP2-72828JF549
Janelia Fluor 646
NBP2-72828JF646
PE
NBP2-72828PE
PerCP
NBP2-72828PCP
mFluor Violet 450 SE
NBP2-72828MFV450
mFluor Violet 500 SE
NBP2-72828MFV500
mFluor Violet 610 SE
NBP2-72828MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72828
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Musashi-1 Antibody (OTI1G8) Summary

Immunogen
Full length human recombinant protein of human MSI1 (NP_002433) produced in HEK293T cell.
Marker
Neuronal Stem Cell Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MSI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000-2000
Theoretical MW
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Musashi-1 Antibody (OTI1G8)

  • MSI1
  • Musashi (Drosophila) homolog 1
  • musashi homolog 1 (Drosophila)
  • Musashi1
  • Musashi-1
  • RNA-binding protein Musashi homolog 1

Background

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MSI1
Entrez