![Immunocytochemistry/Immunofluorescence: INT3 Antibody [NBP2-57633] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/INT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57633-img0001.jpg "Immunocytochemistry/Immunofluorescence: INT3 Antibody [NBP2-57633] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LEHLDNLRLNLTNTKQNFFSQTPILQALQHVQASCDEAHKMKFSDLFSLAEEYEDSSTKPPKSRRKAALSSPRSRKNATQP |
| Predicted Species | Mouse (99%), Rat (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | INTS3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC-P, Retrieval method: HIER pH6. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | INTS3 |
![Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human fallopian tube shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/INT3-Antibody-Immunohistochemistry-Paraffin-NBP2-57633-img0002.jpg "Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human fallopian tube shows strong positivity in glandular cells.")
![Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp2-57633_rabbit-polyclonal-int3-antibody-immunohistochemistry-paraffin-1011202311279..jpg "Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.")
![Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human testis shows strong nuclear positivity in spermatogonia.](http://images.novusbio.com/fullsize/antibody/nbp2-57633_rabbit-polyclonal-int3-antibody-immunohistochemistry-paraffin-10112023112830..jpg "Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human testis shows strong nuclear positivity in spermatogonia.")
![Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-57633_rabbit-polyclonal-int3-antibody-immunohistochemistry-paraffin-10112023112931..jpg "Immunohistochemistry-Paraffin: INT3 Antibody [NBP2-57633] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunocytochemistry Notch-4 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af3847_human-notch-4-intracellular-domain-affinity-purified-pab-immunocytochemistry-3092021102949.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")