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Snapin Antibody (809116)

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Snapin Monoclonal Antibody (Catalog # MAB7795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Snapin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Snapin Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
809116
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7795-SP
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Snapin Antibody (809116) Summary

Immunogen
E. coli-derived recombinant human Snapin
Met1-Lys136
Accession # O95295
Specificity
Detects human Snapin in direct ELISAs and human and mouse Snapin in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SNAPIN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Snapin Antibody (809116)

  • SNAP-25-binding protein
  • SNAP25BP
  • SNAPAP
  • SNAPAPSNARE-associated protein Snapin
  • SNAP-associated proteinSNARE associated protein snapin
  • Snapin
  • Synaptosomal-associated protein 25-binding protein

Background

Snapin (SNARE-associated protein) is an approximately 15 kDa, 136 amino acid vesicular phosphoprotein that contains a coiled-coil interacting domain. In neurons, it binds SNAP25 and RGS7 and regulates steps between synaptic vesicle docking and neurotransmitter release. In pancreatic beta cells, it mediates incretin-stimulated insulin secretion. In germ cells, it binds PUM2 and Nanos1 as a putative adaptor protein. Human Snapin shares 98% aa sequence identity with mouse and rat Snapin, while mouse and rat Snapin share 100% aa sequence identity with each other.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SNAPIN
Uniprot