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HARS2 Antibody (OTI5H2) - Azide and BSA Free

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Western Blot: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HARS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Staining of paraffin-embedded Human Ovary tissue using anti-HARS2 mouse monoclonal antibody.
Western Blot: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HARS2 monoclonal antibody.
Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HARS2 mouse monoclonal antibody.
Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HARS2 mouse monoclonal antibody.
Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Staining of paraffin-embedded Human breast tissue using anti-HARS2 mouse monoclonal antibody.
Immunohistochemistry: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - Staining of paraffin-embedded Human tonsil using anti-HARS2 mouse monoclonal antibody.
CyTOF-ready: HARS2 Antibody (OTI5H2) - Azide and BSA Free [NBP2-71739] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HARS2 ...read more

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI5H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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HARS2 Antibody (OTI5H2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human HARS2(NP_036340) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HARS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500-2000
Theoretical MW
53.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HARS2 Antibody (OTI5H2) - Azide and BSA Free

  • EC 6.1.1
  • EC 6.1.1.21
  • HARSLprobable histidyl-tRNA synthetase, mitochondrial
  • HARS-related
  • HARSRhistidine tRNA ligase 2, mitochondrial (putative)
  • HisRS
  • histidine translase
  • Histidine--tRNA ligase
  • Histidine--tRNA ligase-like
  • histidyl-tRNA synthetase 2, mitochondrial (putative)
  • histidyl-tRNA synthetase-like
  • HO3histidine-tRNA ligase homolog

Background

The HARS2 gene encodes a 506 amino acid, 56kDA probable histidine--tRNA ligase (mitochondrial) protein that is member to the II family of aminoacyl-tRNA synthetases which charge tRNA with cognate amino acids. The HARS2 gene is located in a head-to-head orientation with HARS on chromosome 5, which is the location of where homologous genes share a bidirectional promoter. The protein works as an accessory in the regulation of protein biosynthesis through the synthesis of histidyl-transfer RNA. HARS2 is involved in gene expression and mitochondrian tRNA aminoacylation and has been known to interact with genes ICT1, AARS2, AASS, ABSB7, and ACAD9. HARS2 has been investigated in immunodeficiency, malaria, pneumonia, hearing loss, tuberculosis, schizophrenia, and ovarian dysgenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HARS2