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HARS2 Antibody (OTI5H2)

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Western Blot: HARS2 Antibody (5H2) [NBP2-01425] Analysis of extracts (35ug) from 9 different cell lines by using anti-HARS2 monoclonal antibody.
Immunohistochemistry-Paraffin: HARS2 Antibody (5H2) [NBP2-01425] - Staining of paraffin-embedded Human tonsil using anti-HARS2 mouse monoclonal antibody.
Flow Cytometry: HARS2 Antibody (5H2) [NBP2-01425] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HARS2 antibody, and then analyzed ...read more
Western Blot: HARS2 Antibody (5H2) [NBP2-01425] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HARS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: HARS2 Antibody (5H2) [NBP2-01425] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HARS2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HARS2 Antibody (5H2) [NBP2-01425] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HARS2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HARS2 Antibody (5H2) [NBP2-01425] - Staining of paraffin-embedded Human breast tissue using anti-HARS2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HARS2 Antibody (5H2) [NBP2-01425] - Staining of paraffin-embedded Human Ovary tissue using anti-HARS2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI5H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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HARS2 Antibody (OTI5H2) Summary

Immunogen
Full length human recombinant protein of human HARS2(NP_036340) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HARS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
53.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HARS2 Antibody (OTI5H2)

  • EC 6.1.1
  • EC 6.1.1.21
  • HARSLprobable histidyl-tRNA synthetase, mitochondrial
  • HARS-related
  • HARSRhistidine tRNA ligase 2, mitochondrial (putative)
  • HisRS
  • histidine translase
  • Histidine--tRNA ligase
  • Histidine--tRNA ligase-like
  • histidyl-tRNA synthetase 2, mitochondrial (putative)
  • histidyl-tRNA synthetase-like
  • HO3histidine-tRNA ligase homolog

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes share a bidirectional promoter. [provided by RefSeq]. COMPLETENESS: full length.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HARS2
Entrez