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YARS Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86889] - Staining in human cerebral cortex and skeletal muscle tissues using NBP1-86889 antibody. Corresponding YARS RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86889] - Staining of human cerebral cortex, pancreas, skeletal muscle and testis using Anti-YARS antibody NBP1-86889 (A) shows similar ...read more
Genetic Strategies: Western Blot: YARS Antibody [NBP1-86889] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-YARS antibody. Remaining relative intensity ...read more
Immunocytochemistry/ Immunofluorescence: YARS Antibody [NBP1-86889] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Western Blot: YARS Antibody [NBP1-86889] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86889] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86889] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86889] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86889] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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YARS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPMAKGPAKNSEPEEVIPSRLDIRVGKIITVEKHPDAD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YARS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
YARS Protein (NBP1-86889PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for YARS Antibody

  • CMTDIC
  • EC 6.1.1
  • EC 6.1.1.1
  • Tyrosyl--tRNA ligase
  • tyrosyl-tRNA synthetase
  • tyrosyl-tRNA synthetase, cytoplasmic
  • TYRRS
  • YRStyrRS
  • YTStyrosine tRNA ligase 1, cytoplasmic

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YARS1