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GAP-43 Antibody

Images

 
Western Blot: GAP-43 Antibody [NB100-81934] - Analysis of extract from mouse brain tissue, using NB100-81934 and the same antibody preincubated with blocking peptide.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB100-81934] - Staining of methanol-fixed Hela cells using NB100-81934
Immunohistochemistry-Paraffin: GAP-43 Antibody [NB100-81934] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81934 (left) or the same antibody preincubated with blocking peptide (right).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GAP-43 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human GAP-43 around amino acids 38~43 (Q-A-S-F-R).
Marker
Neuronal Marker
Specificity
Detects endogenous levels of total GAP-43 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAP43
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAP-43 Antibody

  • Axonal membrane protein GAP-43
  • B-50
  • Basp2
  • calmodulin-binding protein P-57
  • GAP43
  • GAP-43
  • growth associated protein 43
  • Growth-associated protein 43
  • nerve growth-related peptide GAP43
  • Neural phosphoprotein B-50
  • Neuromodulin
  • neuron growth-associated protein 43
  • PP46
  • protein F1

Background

GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GAP-43 Antibody (NB100-81934) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on GAP-43.

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Bioinformatics

Gene Symbol GAP43
Uniprot