![Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Paraffin-NB300-143-img0019.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH. |
| Localization | Neuronal Marker, developing and regenerating axons and growth cone. |
| Marker | Neuronal Marker |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GAP43 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, and Western blot, where it recognizes a band at 43 kDa. Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 29950987 and 27007292 respectively). Use in FLOW reported in scientific literature (PMID: 21614130). |
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| Theoretical MW | 43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Publications using NB300-143 | Applications | Species |
|---|---|---|
| Shayya H The unfolded protein response couples neuronal identity to circuit formation in the developing mouse olfactory system Thesis 2023-01-01 (IF/IHC, Mouse) | IF/IHC | Mouse |
| Cain MD, Klein NR, Jiang X, Klein RS. Post-exposure intranasal IFN? suppresses replication and neuroinvasion of Venezuelan Equine Encephalitis virus within olfactory sensory neurons bioRxiv 2023-07-07 [PMID: 37425867] (ICC/IF, IHC) | ICC/IF, IHC | |
| Huang SWJ Neurogenesis in the Olfactory System: The Functional Activity of Immature Olfactory Sensory Neurons and Postnatal-Born Granule Cells Thesis 2022-01-01 (IHC-Fr, Mouse) | IHC-Fr | Mouse |
| Zhang Y Morphogenetic roles of Epb41l5 and cell heterogeneity in early kidney development Thesis 2022-01-01 (IHC-P, Mouse) Details: Dilution used for IHC 1:200 |
IHC-P | Mouse |
| Hanell M Optimization of protocol for immunofluorescence stain to observe nerve infiltration and regeneration in cancer tissue Thesis 2022-01-01 | ||
| Balakrishnan A Glial cells in health and disease Thesis 2020-01-01 (ICC/IF, Mouse) | ICC/IF | Mouse |
| Acton L Investigating Mechanisms Responsible for the Pro-Regenerative Effects of Conditioning Electrical Stimulation Thesis 2022-01-01 (IHC-Fr, Mouse) Details: Adult 129S6/SvEvTac (wildtype) mice, IHC-Fr dilution used 1:500 |
IHC-Fr | Mouse |
| Tamaru T, Kobayakawa K, Saiwai H et al. Glial scar survives until the chronic phase by recruiting scar-forming astrocytes after spinal cord injury Experimental neurology 2023-01-01 [PMID: 36336030] (IHC-Fr, Mouse) Details: Dilution used in IHC-Fr 1:500 |
IHC-Fr | Mouse |
| Huang JS, Kunkhyen T, Rangel AN et al. Immature olfactory sensory neurons provide behaviourally relevant sensory input to the olfactory bulb Nature communications 2022-10-19 [PMID: 36261441] (IHC-Fr, Mouse) Details: Dilution used in IHC 1:1000 |
IHC-Fr | Mouse |
| Efraim Y, Chen FYT, Cheong KN et al. A synthetic tear protein resolves dry eye through promoting corneal nerve regeneration Cell reports 2022-08-30 [PMID: 36044852] (IF/IHC, Mouse) Details: Dilutions: 1:400 |
IF/IHC | Mouse |
| Show All 94 Publications. | ||
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Bryan Tinsley |
IHC-P | Mouse | 11/07/2013 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GAP-43 Antibody (NB300-143)Find related products by research area.
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Synapsin I: Implicated in synaptic activity across a diverse range of studies Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal. Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i... Read full blog post. |
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| Gene Symbol | GAP43 |
![Western Blot: GAP-43 Antibody [NB300-143] - Different tissue and cell lysates using rabbit pAb to GAP43, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NB300-143-img0022.jpg "Western Blot: GAP-43 Antibody [NB300-143] - Different tissue and cell lysates using rabbit pAb to GAP43, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein")
![Immunohistochemistry: GAP-43 Antibody [NB300-143] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Citeab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-NB300-143-img0024.jpg "Immunohistochemistry: GAP-43 Antibody [NB300-143] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Citeab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Posterior MOE sections at approximately the same region where EOG was recorded were obtained from control and Skn-1a-/- mice exposed to either water or chemicals for 2 wks. The sections were immunoreacted with antibodies against GAP43 and OMP and stained with DAPI. Confocal images were taken from olfactory turbinate II. Similar morphology and marker expression were found in four groups of mice, indicating there was no obvious tissue damage in both control and Skn-1a-/- mice after chemical exposure in the posterior MOE regions where EOG recordings were performed. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//eneuro.sfn.org/lookup/doi/10.1523/ENEURO.0135-17.2017), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Paraffin-NB300-143-img0023.jpg "Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Posterior MOE sections at approximately the same region where EOG was recorded were obtained from control and Skn-1a-/- mice exposed to either water or chemicals for 2 wks. The sections were immunoreacted with antibodies against GAP43 and OMP and stained with DAPI. Confocal images were taken from olfactory turbinate II. Similar morphology and marker expression were found in four groups of mice, indicating there was no obvious tissue damage in both control and Skn-1a-/- mice after chemical exposure in the posterior MOE regions where EOG recordings were performed. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//eneuro.sfn.org/lookup/doi/10.1523/ENEURO.0135-17.2017), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, dilution 1:2,000 in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0021.jpg "Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, dilution 1:2,000 in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Rat E18 mixed neuron/glia cultures with rabbit GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0018.jpg "Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Rat E18 mixed neuron/glia cultures with rabbit GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).")
![Western Blot: GAP-43 Antibody [NB300-143] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43 . A prominent band running at ~43kDa represents the full length GAP-43.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NB300-143-img0020.jpg "Western Blot: GAP-43 Antibody [NB300-143] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43 . A prominent band running at ~43kDa represents the full length GAP-43.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells. (Teo, et al, 2005)](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0015.jpg "Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells. (Teo, et al, 2005)")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Mixed neuron-glial cultures stained with RPCA-GAP43 (red), blue is DNA staining.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NB300-143-img0017.jpg "Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Mixed neuron-glial cultures stained with RPCA-GAP43 (red), blue is DNA staining.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
