![Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)](http://images.novusbio.com/fullsize/Dopamine-D1R-DRD1-Antibody-SG2-D1a-Western-Blot-NB110-60017-img0005.jpg "Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)")
| Reactivity | Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)Species Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clone | SG2-D1a |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901] |
| Localization | Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. |
| Specificity | This antibody is specific for Dopamine Receptor D1A. There is no cross-reactivity with D1B (D5) receptor. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DRD1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IF | Rat | 04/05/2016 |
Summary
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reviewed by:
Verified Customer |
ICC | Other | 04/10/2014 |
Summary
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reviewed by:
Verified Customer |
IHC-Fr | Rat | 01/18/2010 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Dopamine D1R/DRD1 Antibody (NB110-60017)Find related products by research area.
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| Verified Customer 04/05/2016 |
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| Application: | IF | |
| Species: | Rat |
| Verified Customer 04/10/2014 |
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| Application: | ICC | |
| Species: | Other |
| Verified Customer 01/18/2010 |
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| Application: | IHC-Fr | |
| Species: | Rat |
| Gene Symbol | DRD1 |
![Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms11829), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Dopamine-D1R-DRD1-Antibody-SG2-D1a-Immunohistochemistry-NB110-60017-img0007.jpg "Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms11829), licensed under a CC-BY license.")
![Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review.](http://images.novusbio.com/fullsize/Dopamine-D1R-DRD1-Antibody-SG2-D1a-Immunohistochemistry-NB110-60017-img0003.jpg "Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review.")
![Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine D1 R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Dopamine-D1R-DRD1-Antibody-SG2-D1a-Immunohistochemistry-Free-Floating-NB110-60017-img0006.jpg "Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine D1 R antibody. Image from verified customer review.")
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