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ASCL1/Mash1 Antibody [Unconjugated]

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ASCL1/Mash1 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS) using Goat Anti-Mouse ASCL1/Mash1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2567) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
ASCL1/Mash1 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c., cross-section through developing CNS) using Goat Anti-Mouse ASCL1/Mash1 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2567
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2567-SP
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ASCL1/Mash1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse ASCL1/Mash1
His74-Asp173 (Gln160Glu)
Accession # Q53WV9
Specificity
Detects mouse ASCL1/Mash1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ASCL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2567 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASCL1/Mash1 Antibody [Unconjugated]

  • achaete-scute complex (Drosophila) homolog-like 1
  • achaete-scute complex homolog 1 (Drosophila)
  • achaete-scute complex-like 1 (Drosophila)
  • achaete-scute homolog 1
  • ASCL1
  • ASH1
  • ASH-1
  • ASH1MASH1
  • BHLHA46
  • bHLHa46achaete-scute complex-like 1
  • Class A basic helix-loop-helix protein 46
  • HASH1
  • HASH1achaete scute protein
  • Mash1

Background

Mash1, also known as achaete-scute complex-like 1 (ASCL1), is a member of the basic helix-loop-helix family of transcription factors. It is required for neuronal commitment and differentiation. The mouse immunogen used to generate this antibody shares 97% amino acid sequence identity with the equivalent region of human Mash1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASCL1/Mash1 Antibody (AF2567)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


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(2)
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Bioinformatics

Gene Symbol ASCL1
Uniprot