| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33376SS | |||
| BSA Free | NBP2-33376-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-74751-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-74753-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20167-0.1mg | |||
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - The indicated amounts of the BioRad Precision Plus Dual Color and All Blue protein standards were separated by SDS-PAGE on a 4-15% gel, transferred to PVDF membrane. The membrane was blocked for 1 hour in 5% non-fat milk in TBST and then incubated with 1.0 ug/mL of Anti-blue antibody and a 1:10,000 dilution of Anti-mouse HRP in 1% non-fat milk TBST for 1 hour. After washing the membrane was processed for chemiluminescent detection. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0004.jpg "Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - The indicated amounts of the BioRad Precision Plus Dual Color and All Blue protein standards were separated by SDS-PAGE on a 4-15% gel, transferred to PVDF membrane. The membrane was blocked for 1 hour in 5% non-fat milk in TBST and then incubated with 1.0 ug/mL of Anti-blue antibody and a 1:10,000 dilution of Anti-mouse HRP in 1% non-fat milk TBST for 1 hour. After washing the membrane was processed for chemiluminescent detection. Image from the standard format of this antibody.")
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Analysis using the HRP conjugate of NBP2-33376. Detection of the location of each of the protein standards is hand marked on the X-ray film or image after exposure. This can lead to errors and inaccuracy in determining the molecular weight and identity of your target protein (Tubulin and Actin are shown as examples in these images). By using the Anti-Blue antibody in conjunction with your target protein antibodies, you can image the complete molecular weight standard and your target protein at the same time. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0001.jpg)
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Analysis using the HRP conjugate of NBP2-33376. Precision Plus Dual Color molecular weight markers (1 and 2 ul) were separated by SDS-PAGE on a 4-14% gel and transferred to PVDF membrane. The membrane was incubated with HRP conjugated Blue Marker Antibody at 1 ug/ml for 30 min. After washing, the membrane was exposed to ECL and the blue markers detected with a 5 second exposure. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0002.jpg)
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Highly specific to blue prestained markers. Does not cross react with whole cell proteins of different species. Whole cell lysate from various cell lines of human, mouse, rat and hamster origin were separated on a 4-15% gel by electrophoresis and transferred to PVDF. The membrane was stained with Amido black to show the presence of protein from all lysates. After blocking in 5% non-fat milk, the membrane was incubated with Anti-Blue at 1 ug/ml. After washing and incubation with Anti-mouse HRP the blot was incubated in ECL reagent and imaged. Note that only the blue molecular weight markers were detected with the Anti-Blue antibody demonstrating its high specificity and suitability for addition to your Western blot procedure. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0003.jpg)
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Works with all prestained blue markers. Prestained molecular weight markers (2 and 5 ul) from three suppliers, BioRad, Invitrogen and GenDepot were separated by electrophoresis on a 4-15% gel and transferred to PVDF membrane. After blocking in 5% non-fat milk the blot was probed with 1 ug/ml Anti-Blue, washed and incubated with an Anti-mouse HRP conjugated secondary antibody followed by ECL incubation. The composite figure shows the visible image of the markers on the blot and its corresponding Western blot image. Note that only the blue protein markers from each supplier are detected by Western blot. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0005.jpg)
![Immunohistochemistry-Paraffin: Olfactory Marker Protein Antibody [NB110-74753] - IHC on rat olfactory using Rabbit antibody to rat & mouse OMP (Olfactory Marker Protein): IgG (NB110-74753) at a concentration of 20 ug/ml in paraffin embeded section. Glomeruli are intensely stained. H&E counter stained.](http://images.novusbio.com/fullsize/Olfactory-Marker-Protein-Antibody-Immunohistochemistry-Paraffin-NB110-74753-img0001.jpg "Immunohistochemistry-Paraffin: Olfactory Marker Protein Antibody [NB110-74753] - IHC on rat olfactory using Rabbit antibody to rat & mouse OMP (Olfactory Marker Protein): IgG (NB110-74753) at a concentration of 20 ug/ml in paraffin embeded section. Glomeruli are intensely stained. H&E counter stained.")
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Mouse embryonic fibroblasts, fixed with 4% PFA washed with PBS, permeabilized with 0.2% Triton X-100. Primary antibody at 1:100 in 3% BSA in PBS. Secondary antibodies incubation for 2 hours. Image from a verified customer review.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0007.jpg "Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Mouse embryonic fibroblasts, fixed with 4% PFA washed with PBS, permeabilized with 0.2% Triton X-100. Primary antibody at 1:100 in 3% BSA in PBS. Secondary antibodies incubation for 2 hours. Image from a verified customer review.")
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0006.jpg)
![Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Western-Blot-NB110-89724-img0018.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89724-img0020.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NB110-89724-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89724-img0019.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord (free floating sections) at 1:1000 dilution using Rabbit antibody to c-terminal region of ChAT (choline O-acetyltransferase): whole serum (NB110-89724).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0013.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0012.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0014.jpg)
![Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Free-Floating-NB110-89724-img0015.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NB110-89724-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB100-98717] - Rat brain at 1:500 dilution using Sheep antibody to rat, mouse Mtap2 whole serum DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/MAP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98717-img0003.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody [NB100-98717] - Rat brain at 1:500 dilution using Sheep antibody to rat, mouse Mtap2 whole serum DAPI counterstained appearing in blue.")
![Western Blot: D4S234E Antibody [NBP2-26148] - NBP2-26148 (0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/D4S234E-Antibody-Western-Blot-NBP2-26148-img0001.jpg "Western Blot: D4S234E Antibody [NBP2-26148] - NBP2-26148 (0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: D4S234E Antibody [NBP2-26148] - (3ug/ml) staining of primary DIV9 cells from Hippocampus E18 Rat embryos showed exclusive localization (red, Alexa 568) within the neurons (MAP2 staining in blue, Alexa 647) and not in the glia (GFAP staining in green, Alexa 488).Right panel shows](http://images.novusbio.com/fullsize/D4S234E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26148-img0002.jpg)