![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - The indicated amounts of the BioRad Precision Plus Dual Color and All Blue protein standards were separated by SDS-PAGE on a 4-15% gel, transferred to PVDF membrane. The membrane was blocked for 1 hour in 5% non-fat milk in TBST and then incubated with 1.0 ug/mL of Anti-blue antibody and a 1:10,000 dilution of Anti-mouse HRP in 1% non-fat milk TBST for 1 hour. After washing the membrane was processed for chemiluminescent detection. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0004.jpg "Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - The indicated amounts of the BioRad Precision Plus Dual Color and All Blue protein standards were separated by SDS-PAGE on a 4-15% gel, transferred to PVDF membrane. The membrane was blocked for 1 hour in 5% non-fat milk in TBST and then incubated with 1.0 ug/mL of Anti-blue antibody and a 1:10,000 dilution of Anti-mouse HRP in 1% non-fat milk TBST for 1 hour. After washing the membrane was processed for chemiluminescent detection. Image from the standard format of this antibody.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB |
| Clone | 6F4-F6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Blue dye stained proteins |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Blue Marker Antibody (6F4-F6) is useful for detecting blue prestained markers in Western blot. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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Blue Marker Antibody - a powerful tool for visualizing markers and target protein simultaneously! Western blotting (or immunoblotting) is a widely used procedure to detect specific proteins in tissues and cell extracts and the Blue Marker Antibody can help make it easier and more accurate to size proteins of interest. The Blue Marker Antibody ... Read full blog post. |
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Blue Marker Antibody: An easy solution for BLUE-pre-stained markers Western blotting is a widely used technique for the detection and analysis of proteins based on their ability to bind to specific antibodies. It was first described by Towbin, et.al in 1979 and has since become one of the most commonly used methods in... Read full blog post. |
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![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Analysis using the HRP conjugate of NBP2-33376. Detection of the location of each of the protein standards is hand marked on the X-ray film or image after exposure. This can lead to errors and inaccuracy in determining the molecular weight and identity of your target protein (Tubulin and Actin are shown as examples in these images). By using the Anti-Blue antibody in conjunction with your target protein antibodies, you can image the complete molecular weight standard and your target protein at the same time. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0001.jpg "Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Analysis using the HRP conjugate of NBP2-33376. Detection of the location of each of the protein standards is hand marked on the X-ray film or image after exposure. This can lead to errors and inaccuracy in determining the molecular weight and identity of your target protein (Tubulin and Actin are shown as examples in these images). By using the Anti-Blue antibody in conjunction with your target protein antibodies, you can image the complete molecular weight standard and your target protein at the same time. Image from the standard format of this antibody.")
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Analysis using the HRP conjugate of NBP2-33376. Precision Plus Dual Color molecular weight markers (1 and 2 ul) were separated by SDS-PAGE on a 4-14% gel and transferred to PVDF membrane. The membrane was incubated with HRP conjugated Blue Marker Antibody at 1 ug/ml for 30 min. After washing, the membrane was exposed to ECL and the blue markers detected with a 5 second exposure. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0002.jpg "Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Analysis using the HRP conjugate of NBP2-33376. Precision Plus Dual Color molecular weight markers (1 and 2 ul) were separated by SDS-PAGE on a 4-14% gel and transferred to PVDF membrane. The membrane was incubated with HRP conjugated Blue Marker Antibody at 1 ug/ml for 30 min. After washing, the membrane was exposed to ECL and the blue markers detected with a 5 second exposure. Image from the standard format of this antibody.")
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Highly specific to blue prestained markers. Does not cross react with whole cell proteins of different species. Whole cell lysate from various cell lines of human, mouse, rat and hamster origin were separated on a 4-15% gel by electrophoresis and transferred to PVDF. The membrane was stained with Amido black to show the presence of protein from all lysates. After blocking in 5% non-fat milk, the membrane was incubated with Anti-Blue at 1 ug/ml. After washing and incubation with Anti-mouse HRP the blot was incubated in ECL reagent and imaged. Note that only the blue molecular weight markers were detected with the Anti-Blue antibody demonstrating its high specificity and suitability for addition to your Western blot procedure. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0003.jpg "Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Highly specific to blue prestained markers. Does not cross react with whole cell proteins of different species. Whole cell lysate from various cell lines of human, mouse, rat and hamster origin were separated on a 4-15% gel by electrophoresis and transferred to PVDF. The membrane was stained with Amido black to show the presence of protein from all lysates. After blocking in 5% non-fat milk, the membrane was incubated with Anti-Blue at 1 ug/ml. After washing and incubation with Anti-mouse HRP the blot was incubated in ECL reagent and imaged. Note that only the blue molecular weight markers were detected with the Anti-Blue antibody demonstrating its high specificity and suitability for addition to your Western blot procedure. Image from the standard format of this antibody.")
![Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Works with all prestained blue markers. Prestained molecular weight markers (2 and 5 ul) from three suppliers, BioRad, Invitrogen and GenDepot were separated by electrophoresis on a 4-15% gel and transferred to PVDF membrane. After blocking in 5% non-fat milk the blot was probed with 1 ug/ml Anti-Blue, washed and incubated with an Anti-mouse HRP conjugated secondary antibody followed by ECL incubation. The composite figure shows the visible image of the markers on the blot and its corresponding Western blot image. Note that only the blue protein markers from each supplier are detected by Western blot. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-Azide-and-BSA-Free-Western-Blot-NBP2-80598-img0005.jpg "Western Blot: Blue Marker Antibody (6F4-F6) - Azide and BSA Free [NBP2-80598] - Works with all prestained blue markers. Prestained molecular weight markers (2 and 5 ul) from three suppliers, BioRad, Invitrogen and GenDepot were separated by electrophoresis on a 4-15% gel and transferred to PVDF membrane. After blocking in 5% non-fat milk the blot was probed with 1 ug/ml Anti-Blue, washed and incubated with an Anti-mouse HRP conjugated secondary antibody followed by ECL incubation. The composite figure shows the visible image of the markers on the blot and its corresponding Western blot image. Note that only the blue protein markers from each supplier are detected by Western blot. Image from the standard format of this antibody.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")