![Western Blot: Blue Marker Antibody (6F4-F6) [HRP] [NBP2-33376H] - In traditional Western blot analysis the location of each of the protein standards is hand marked on the X-ray film or image after exposure. This can lead to errors and inaccuracy in determining the molecular weight and identity of your target protein (Tubulin and Actin are shown as examples in these images). By using the Anti-Blue antibody in conjunction with your target protein antibodies, you can image the complete molecular weight standard and your target protein at the same time.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-HRP-Western-Blot-NBP2-33376H-img0002.jpg "Western Blot: Blue Marker Antibody (6F4-F6) [HRP] [NBP2-33376H] - In traditional Western blot analysis the location of each of the protein standards is hand marked on the X-ray film or image after exposure. This can lead to errors and inaccuracy in determining the molecular weight and identity of your target protein (Tubulin and Actin are shown as examples in these images). By using the Anti-Blue antibody in conjunction with your target protein antibodies, you can image the complete molecular weight standard and your target protein at the same time.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB |
| Clone | 6F4-F6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | HRP |
| Immunogen | Blue dye stained proteins |
| Specificity | Detects BLUE prestained molecular weight markers from different vendors, does not cross-react with whole cell proteins of different species, does not interfere with the detection by other antibodies. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Blue Marker Antibody (6F4-F6) is useful for detecting blue prestained markers in Western blot. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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Blue Marker Antibody - a powerful tool for visualizing markers and target protein simultaneously! Western blotting (or immunoblotting) is a widely used procedure to detect specific proteins in tissues and cell extracts and the Blue Marker Antibody can help make it easier and more accurate to size proteins of interest. The Blue Marker Antibody ... Read full blog post. |
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Blue Marker Antibody: An easy solution for BLUE-pre-stained markers Western blotting is a widely used technique for the detection and analysis of proteins based on their ability to bind to specific antibodies. It was first described by Towbin, et.al in 1979 and has since become one of the most commonly used methods in... Read full blog post. |
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![Western Blot: Blue Marker Antibody (6F4-F6) [HRP] [NBP2-33376H] - Precision Plus Dual Color molecular weight markers (1 and 2 ul) were separated by SDS-PAGE on a 4-14% gel and transferred to PVDF membrane. The membrane was incubated with HRP conjugated Blue Marker Antibody at 1 ug/ml for 30 min. After washing, the membrane was exposed to ECL and the blue markers detected with a 5 second exposure.](http://images.novusbio.com/fullsize/Blue-Marker-Antibody-6F4-F6-HRP-Western-Blot-NBP2-33376H-img0001.jpg "Western Blot: Blue Marker Antibody (6F4-F6) [HRP] [NBP2-33376H] - Precision Plus Dual Color molecular weight markers (1 and 2 ul) were separated by SDS-PAGE on a 4-14% gel and transferred to PVDF membrane. The membrane was incubated with HRP conjugated Blue Marker Antibody at 1 ug/ml for 30 min. After washing, the membrane was exposed to ECL and the blue markers detected with a 5 second exposure.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")