![Western Blot: D4S234E Antibody [NBP2-26148] - NBP2-26148 (0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/D4S234E-Antibody-Western-Blot-NBP2-26148-img0001.jpg "Western Blot: D4S234E Antibody [NBP2-26148] - NBP2-26148 (0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Mu, Rt, Hu, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence EKGTKQPLLEDGFD-C corresponding to N-Terminus according to NP_077042.2. |
| Predicted Species | Human (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | NSG1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western blot: Approx 22kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9kDa according to NP_035072.2). Primary incubation was 1 hour. Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
| Publication using NBP2-26148 | Applications | Species |
|---|---|---|
| Yap CC, Wisco D, Kujala P et al. The somatodendritic endosomal regulator NEEP21 facilitates axonal targeting of L1/NgCAM. J Cell Biol 2008-02-25 [PMID: 18299352] |
Secondary Antibodies |
Isotype Controls |
Research Areas for D4S234E Antibody (NBP2-26148)Find related products by research area.
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| Gene Symbol | NSG1 |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: D4S234E Antibody [NBP2-26148] - (3ug/ml) staining of primary DIV9 cells from Hippocampus E18 Rat embryos showed exclusive localization (red, Alexa 568) within the neurons (MAP2 staining in blue, Alexa 647) and not in the glia (GFAP staining in green, Alexa 488).Right panel shows](http://images.novusbio.com/fullsize/D4S234E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26148-img0002.jpg "Immunocytochemistry/Immunofluorescence: D4S234E Antibody [NBP2-26148] - (3ug/ml) staining of primary DIV9 cells from Hippocampus E18 Rat embryos showed exclusive localization (red, Alexa 568) within the neurons (MAP2 staining in blue, Alexa 647) and not in the glia (GFAP staining in green, Alexa 488).Right panel shows")
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