![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex, colon, kidney and testis using Anti-TGOLN2 antibody NBP1-86949 (A) shows similar protein distribution across tissues to independent antibody NBP1-86948 (B).](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0009.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex, colon, kidney and testis using Anti-TGOLN2 antibody NBP1-86949 (A) shows similar protein distribution across tissues to independent antibody NBP1-86948 (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: ATESVKQEEAGVRPSAGNVSTHPSLSQRPGGSTKSHPEPQTPKDSPSKSSAEAQTPEDTPNKSGAEAKTQKDSSNRSGA |
| Marker | TGN Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TGOLN2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-86949 | Applications | Species |
|---|---|---|
| Philippe M, Leger T, Desvaux R, Walch L. Discs Large 1 (Dlg1) Scaffolding Protein Participates with Clathrin and Adaptator Protein Complex 1 (AP-1) in Forming Weibel-Palade Bodies of Endothelial Cells. J Biol Chem 2013-05-03 [PMID: 23532850] (IP, WB, ICC/IF, Human) | IP, WB, ICC/IF | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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-(01-ml)_NBP1-86949_9901.jpg)
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reviewed by:
Verified Customer |
WB | Human | 09/05/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for TGN46 Antibody (NBP1-86949)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TGOLN2 |
![Western Blot: TGN46 Antibody [NBP1-86949] - Analysis in control (vector only transfected HEK293T lysate) and TGOLN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-86949-img0008.jpg "Western Blot: TGN46 Antibody [NBP1-86949] - Analysis in control (vector only transfected HEK293T lysate) and TGOLN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-86949] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86949-img0007.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-86949] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0016.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.")
![Western Blot: TGN46 Antibody [NBP1-86949] - Analysis of lysate from 293T cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-86949-img0006.jpg "Western Blot: TGN46 Antibody [NBP1-86949] - Analysis of lysate from 293T cells. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0010.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human kidney.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0011.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human kidney.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0012.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human testis.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0013.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human testis.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human colon.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0015.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human colon.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0014.jpg "Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP1-86949_9901.jpg)