TGN46 Antibodies
We offer TGN46 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each TGN46 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TGN46 Antibodies can be used in a variety of model species: Human. Use the list below to choose the TGN46 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TGN46 polyclonal antibodies and browse our TGN46 monoclonal antibody catalog.
Alternate Names for TGN46 Antibodies
anti-TGN46 antibody, anti-TGOLN2 antibody, anti-TGN 46 antibody, anti-TGN 48 antibody, anti-TGN 51 antibody, anti-TGN-38 antibody, anti-TGN-46 antibody, anti-TGN38 antibody, anti-TGN38 homolog antibody, anti-TGN48 antibody, anti-TGN51 antibody, anti-TGOLN 2 antibody, anti-trans golgi network 38 antibody, anti-trans golgi network 46 antibody, anti-Trans Golgi network integral membrane protein 2 [Precursor] antibody, anti-Trans Golgi network integral membrane protein 2 antibody, anti-Trans Golgi network protein (46 48 51kD isoforms) antibody, anti-Trans golgi network protein 2 antibody, anti-Trans Golgi network protein TGN51 antibody, anti-Trans-Golgi network integral membrane protein 2 antibody, anti-Trans-Golgi network protein 2 antibody, anti-Trans-Golgi network protein TGN51 antibody, anti-TTGN 2 antibody, anti-TTGN2 antibody
6 Results for "tgn46" in Primary Antibodies
![Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human TGN46 Polyclonal Antibody (NBP1-49643) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for TGN46 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TGN46-Antibody-Knockout-Validated-NBP1-49643-img0017.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human TGN46 Polyclonal Antibody (NBP1-49643) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for TGN46 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - A7 cells expressing F-NTPase were fixed and dual stained with an anti-FLAG antibody (green) and TGN46 antibody (red). Colocalization of F-NTPase with the signals of organelle-specific markers are shown in yellow (arrows). The dashed boxes in each merged image were enlarged and are shown at the right. As noted, the nonvesicular areas of F-NTPase does not colocalize with the signals of TGN46 (a trans-Golgi marker). Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//jvi.asm.org/content/92/5/e01824-17) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - U-2 OS cells were transfected with siRNAs targeting UGCG (siUGCG) or a non-targeting control (siNegCtrl) and plated onto glass coverslips. At 72 hours post-transfection cells were chilled to 4 degrees C on ice and rVSV-SFTSV was bound by centrifugation (1200xg, 30', 4 degrees C). Following centrifugation, media was replaced with pre-warmed media (37 degrees C) and the cells placed in a 37 degrees C incubator for 40 minutes before fixation in 1% paraformaldehyde for 15 minutes. Cells were then immunostained for viral antigen (anti-VSV M, red), TGN46 (green), and nuclei stained with DAPI (blue). Images are representative from at least 3 independent experiments. Boxes indicate zoomed-in regions. Scale bar represents 5um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006316) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0022.jpg)
![Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Analysis of TGOLN2 in human renal cell carcinoma.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-NBP1-49643-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0023.jpg)
![Flow Cytometry: TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/TGN46-Antibody-Flow-Cytometry-NBP1-49643-img0018.jpg)
![Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-49643-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - Analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, blue). Image from the Alexa Fluor 488 version of this antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0019.jpg)
![Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/TGN46-Antibody-Flow-Intracellular-NBP1-49643-img0013.jpg)
![Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TGN46-Antibody-Flow-Intracellular-NBP1-49643-img0014.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex, colon, kidney and testis using Anti-TGOLN2 antibody NBP1-86949 (A) shows similar protein distribution across tissues to independent antibody NBP1-86948 (B).](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex, colon, kidney and testis using Anti-TGOLN2 antibody NBP1-86949 (A) shows similar protein distribution across tissues to independent antibody NBP1-86948 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: TGN46 Antibody [NBP1-86949] - Analysis in control (vector only transfected HEK293T lysate) and TGOLN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-86949-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-86949] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86949-img0007.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0016.jpg)
![Western Blot: TGN46 Antibody [NBP1-86949] - Analysis of lysate from 293T cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-86949-img0006.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0010.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human kidney.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0011.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0012.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human testis.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0013.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human colon.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0015.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86949-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TGN46-Antibody-2F11-Immunocytochemistry-Immunofluorescence-H00010618-M02-img0003.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: TGN46 Antibody (2F11) [H00010618-M02] - Analysis of monoclonal antibody to TGOLN2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TGN46-Antibody-2F11-Immunohistochemistry-Paraffin-H00010618-M02-img0005.jpg)
![ELISA: TGN46 Antibody (2F11) [H00010618-M02] - Detection limit for recombinant GST tagged TGOLN2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TGN46-Antibody-2F11-ELISA-H00010618-M02-img0007.jpg)
![Western Blot: TGN46 Antibody [NBP1-86948] - Analysis in control (vector only transfected HEK293T lysate) and TGOLN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-86948-img0009.jpg "Western Blot: TGN46 Antibody [NBP1-86948] - Analysis in control (vector only transfected HEK293T lysate) and TGOLN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-86948] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86948-img0008.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86948] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86948-img0013.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86948] - Staining of human stomach shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86948-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86948] - Staining of human cerebral cortex, colon, kidney and testis using Anti-TGOLN2 antibody NBP1-86948 (A) shows similar protein distribution across tissues to independent antibody NBP1-86949 (B).](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86948-img0010.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86948] - Staining of human kidney.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86948-img0011.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86948] - Staining of human testis.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86948-img0012.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86948] - Staining of human colon.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-86948-img0014.jpg)
![Western Blot: TGN46 Antibody (9G5X6) [NBP3-15817] - Western blot analysis of extracts of HeLa cells, using TGN46 Rabbit mAb (NBP3-15817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TGN46-Antibody-9G5X6-Western-Blot-NBP3-15817-img0003.jpg "Western Blot: TGN46 Antibody (9G5X6) [NBP3-15817] - Western blot analysis of extracts of HeLa cells, using TGN46 Rabbit mAb (NBP3-15817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody (9G5X6) [NBP3-15817] - Immunofluorescence analysis of U-2 OS cells using TGN46 Rabbit mAb (NBP3-15817) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TGN46-Antibody-9G5X6-Immunocytochemistry-Immunofluorescence-NBP3-15817-img0002.jpg)
![Immunohistochemistry-Paraffin: TGN46 Antibody (9G5X6) [NBP3-15817] - Immunohistochemistry of paraffin-embedded human liver using TGN46 Rabbit mAb (NBP3-15817) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TGN46-Antibody-9G5X6-Immunohistochemistry-Paraffin-NBP3-15817-img0001.jpg)
![Western Blot: TGN46 Antibody [NBP1-62556] - Titration: 0.2-1 ug/ml, Positive Control: Human Stomach.](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-62556-img0004.jpg "Western Blot: TGN46 Antibody [NBP1-62556] - Titration: 0.2-1 ug/ml, Positive Control: Human Stomach.")
![Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-62556] - Human Liver tissue, 5 ug/ml.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-Paraffin-NBP1-62556-img0003.jpg)