![Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human TGN46 Polyclonal Antibody (NBP1-49643) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for TGN46 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TGN46-Antibody-Knockout-Validated-NBP1-49643-img0017.jpg "Knockout Validated: TGN46 Antibody [NBP1-49643] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human TGN46 Polyclonal Antibody (NBP1-49643) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for TGN46 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
| Reactivity | Hu, Mu, Bv, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, WB, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A genomic peptide made to an internal region of the human TGN46 protein (within residues 200-350). [Swiss-Prot: O43493] |
| Localization | Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes |
| Marker | TGN Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TGOLN2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||
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-(01-ml)_NBP1-49643_8236.bmp)
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reviewed by:
Bryan Tinsley |
ICC | Human | 06/12/2014 |
Summary
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-(01-ml)_NBP1-49643_8231.jpg)
Enlarge |
reviewed by:
Bryan Tinsley |
WB | Human | 06/12/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for TGN46 Antibody (NBP1-49643)Find related products by research area.
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| Bryan Tinsley 06/12/2014 |
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| Application: | ICC | |
| Species: | Human |
| Bryan Tinsley 06/12/2014 |
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| Application: | WB | |
| Species: | Human |
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - A7 cells expressing F-NTPase were fixed and dual stained with an anti-FLAG antibody (green) and TGN46 antibody (red). Colocalization of F-NTPase with the signals of organelle-specific markers are shown in yellow (arrows). The dashed boxes in each merged image were enlarged and are shown at the right. As noted, the nonvesicular areas of F-NTPase does not colocalize with the signals of TGN46 (a trans-Golgi marker). Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//jvi.asm.org/content/92/5/e01824-17) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0021.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - A7 cells expressing F-NTPase were fixed and dual stained with an anti-FLAG antibody (green) and TGN46 antibody (red). Colocalization of F-NTPase with the signals of organelle-specific markers are shown in yellow (arrows). The dashed boxes in each merged image were enlarged and are shown at the right. As noted, the nonvesicular areas of F-NTPase does not colocalize with the signals of TGN46 (a trans-Golgi marker). Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//jvi.asm.org/content/92/5/e01824-17) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - U-2 OS cells were transfected with siRNAs targeting UGCG (siUGCG) or a non-targeting control (siNegCtrl) and plated onto glass coverslips. At 72 hours post-transfection cells were chilled to 4 degrees C on ice and rVSV-SFTSV was bound by centrifugation (1200xg, 30', 4 degrees C). Following centrifugation, media was replaced with pre-warmed media (37 degrees C) and the cells placed in a 37 degrees C incubator for 40 minutes before fixation in 1% paraformaldehyde for 15 minutes. Cells were then immunostained for viral antigen (anti-VSV M, red), TGN46 (green), and nuclei stained with DAPI (blue). Images are representative from at least 3 independent experiments. Boxes indicate zoomed-in regions. Scale bar represents 5um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006316) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0022.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - U-2 OS cells were transfected with siRNAs targeting UGCG (siUGCG) or a non-targeting control (siNegCtrl) and plated onto glass coverslips. At 72 hours post-transfection cells were chilled to 4 degrees C on ice and rVSV-SFTSV was bound by centrifugation (1200xg, 30', 4 degrees C). Following centrifugation, media was replaced with pre-warmed media (37 degrees C) and the cells placed in a 37 degrees C incubator for 40 minutes before fixation in 1% paraformaldehyde for 15 minutes. Cells were then immunostained for viral antigen (anti-VSV M, red), TGN46 (green), and nuclei stained with DAPI (blue). Images are representative from at least 3 independent experiments. Boxes indicate zoomed-in regions. Scale bar represents 5um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006316) licensed under a CC-BY license.")
![Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Analysis of TGOLN2 in human renal cell carcinoma.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunohistochemistry-NBP1-49643-img0006.jpg "Immunohistochemistry: TGN46 Antibody [NBP1-49643] - Analysis of TGOLN2 in human renal cell carcinoma.")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0023.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/TGN46-Antibody-Flow-Cytometry-NBP1-49643-img0018.jpg "Flow Cytometry: TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.")
![Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).](http://images.novusbio.com/fullsize/TGN46-Antibody-Western-Blot-NBP1-49643-img0009.jpg "Western Blot: TGN46 Antibody [NBP1-49643] - Analysis of extracts from HeLa cells using TGN46 antibody (NBP1-49643, 1:100).")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0024.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with TGN46 Antibody conjugated to Alexa Fluor 488 (NBP1-49643AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - Analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0008.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - Analysis of A549 cells using TGN46 antibody (NBP1-49643, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, blue). Image from the Alexa Fluor 488 version of this antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0016.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - T98G glioblastoma cells probed for Golgi (Alexa Fluor 488 conjugated TGN-46 antibody, Green), tubulin (Alexa Fluor 594, Red), and nucleus (DAPI, blue). Image from the Alexa Fluor 488 version of this antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.](http://images.novusbio.com/fullsize/TGN46-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49643-img0019.jpg "Immunocytochemistry/Immunofluorescence: TGN46 Antibody [NBP1-49643] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.")
![Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/TGN46-Antibody-Flow-Intracellular-NBP1-49643-img0013.jpg "Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on U-937 cells with NBP1-49643 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).")
![Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TGN46-Antibody-Flow-Intracellular-NBP1-49643-img0014.jpg "Flow (Intracellular): TGN46 Antibody [NBP1-49643] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg)
![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Immunohistochemistry Cathepsin D Antibody [Unconjugated]](https://images.novusbio.com/images2/Cathepsin_D_AF1029_Immunohistochemistry_6668.jpg)
![Immunocytochemistry Cathepsin D Antibody [Unconjugated]](https://images.novusbio.com/images2/Cathepsin_D_AF1029_Immunocytochemistry__Immunofluorescence_23054.jpg)
![Western Blot Cathepsin D Antibody [Unconjugated]](https://images.novusbio.com/images2/Cathepsin_D_AF1029_Western_Blot_19580.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP1-49643_8236.bmp)
-(01-ml)_NBP1-49643_8231.jpg)