![Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Western-Blot-NBP2-80954-img0002.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review. Image from the standard format of this antibody.")
| Reactivity | Mu, Rt, Hu(-)Species Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clone | mAb16 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977] |
| Epitope | N-terminus |
| Localization | Membrane; Multi-pass membrane protein. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SLC6A3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In WB a band can been seen at ~70-85 kDa. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15 M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SLC6A3/DAT1 Antibody (NBP2-80954)Find related products by research area.
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| Gene Symbol | SLC6A3 |
![Immunocytochemistry/Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80954-img0003.jpg "Immunocytochemistry/Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.")
![Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80954-img0004.jpg "Immunohistochemistry: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.")
![Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Analysis of DAT1 expression in rat striatal membrane tissue lysate. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/SLC6A3-DAT1-Antibody-mAb16-Azide-and-BSA-Free-Western-Blot-NBP2-80954-img0001.jpg "Western Blot: SLC6A3/DAT1 Antibody (mAb16) - Azide and BSA Free [NBP2-80954] - Analysis of DAT1 expression in rat striatal membrane tissue lysate. Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")