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SLC6A3/DAT1 Antibody (CL3123)

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Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody (3123) [NBP2-46649] - Analysis of human pancreas shows absence of immunoreactivity (negative control).
Immunohistochemistry: SLC6A3/DAT1 Antibody (3123) [NBP2-46649] - Staining of rat striatum shows strong immunoreactivity in dopaminergic fibers.
Immunohistochemistry: SLC6A3/DAT1 Antibody (3123) [NBP2-46649] - Staining of mouse striatum shows strong immunoreactivity in dopaminergic fibers.
Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody (3123) [NBP2-46649] - Staining of human caudate nucleus shows immunoreactivity in dopaminergic fibers.
Immunohistochemistry: SLC6A3/DAT1 Antibody (3123) [NBP2-46649] - Staining of rat cerebral cortex shows positivity in dopaminergic fibers.
Immunohistochemistry: SLC6A3/DAT1 Antibody (3123) [NBP2-46649] - Staining of mouse cerebral cortex shows positivity in dopaminergic fibers.
Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody (3123) [NBP2-46649] - Staining of human substantia nigra shows moderate immunoreactivity in dopamine neurons and fibers.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clone
CL3123
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SLC6A3/DAT1 Antibody (CL3123) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence LHYLFSSFTTELPWNNSWNSPNCSDAHPGDSSGDSSGLNDTFGTTPAAEYFERGVLHLHQSHGIDDLG
Epitope
ELPWIHCNNSWNSPN
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC6A3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
SLC6A3/DAT1 Recombinant Protein Antigen (NBP2-46649PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SLC6A3/DAT1 Antibody (CL3123)

  • DA transporter
  • DAT1
  • DATDAT1Solute carrier family 6 member 3
  • SLC6A3
  • sodium-dependent dopamine transporter
  • solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
  • VNTR

Background

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for SLC6A3/DAT1 Antibody (NBP2-46649). (Showing 1 - 1 of 1 FAQ).

  1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
    • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC6A3
Entrez
Uniprot