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Septin-3 Antibody

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Western Blot: Septin-3 Antibody [NB100-1023] - NB100-1023 (0.5ug/ml) staining of Human Cerebellum (A), (0.1ug/ml) Mouse (B) and (0.03ug/ml) Rat (C) Brain lysate (35ug protein in RIPA buffer).. Detected by ...read more
Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Septin-3 Antibody Summary

Immunogen
Peptide with sequence SELVPEPRPKPA-C corresponding to N-Terminus according to NP_663786.2, NP_061979.3.
Specificity
This antibody is expected to recognize both reported isoforms according to NP_663786.2 and NP_061979.3.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SEPT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.5 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 40.7kDa according to NP_663786.2 and NP_062248.2, and 40.6kDa according to NP_001345765.1)
Control
Septin-3 Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-1023.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Septin-3 Antibody

  • bK250D10.3
  • MGC133218
  • neuronal-specific septin-3
  • SEP3neuronal-specific septin 3
  • septin 3

Background

This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Septin-3 Antibody (NB100-1023)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Septin-3 Antibody (NB100-1023) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Septin-3

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Bioinformatics

Gene Symbol SEPT3
Entrez
Uniprot