![Western Blot: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12. Lane 1: SEPT1 transfected lysatE (42 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Septin-1-Antibody-1F12-Western-Blot-H00001731-M03-img0004.jpg "Western Blot: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12. Lane 1: SEPT1 transfected lysatE (42 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 1F12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | SEPT1 (AAH12161, 1 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDKEYVGFAALPNQLHRKSVKKGFDFTLMVAGESGLGKSTLINSLFLTNLYEDRQVPEASARLTQTLAIERRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLPVVKFIEEQFEQYLRDESGLNRKNIQDSRVHCCLYFISPFGRGLRPLDVAFLRAVHEKVNIIPVIGKADALMPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDEDFKRQDAEMKESIPFAVVGSCEVVRDGGNRPVRGRRYSWGTVEVENPHHCDFLNLRRMLVQTHLQDLKEVTHDLLYEGYRARRLQSLARPGARDRASRSKLSRQSATEIPLPMLPLADTEKLIREKDEELRRMQEMLEKMQAQMQRSQAQGEQSDAL |
| Specificity | SEPT1 - septin 1 (1F12) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SEPT1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Septin-1 Antibody (H00001731-M03)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of monoclonal antibody to SEPT1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Septin-1-Antibody-1F12-Immunocytochemistry-Immunofluorescence-H00001731-M03-img0001.jpg "Immunocytochemistry/Immunofluorescence: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of monoclonal antibody to SEPT1 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunoprecipitation: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEPT1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Septin-1-Antibody-1F12-Immunoprecipitation-H00001731-M03-img0002.jpg "Immunoprecipitation: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEPT1 rabbit polyclonal antibody.")
![Sandwich ELISA: Septin-1 Antibody (1F12) [H00001731-M03] - Detection limit for recombinant GST tagged SEPT1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Septin-1-Antibody-1F12-Sandwich-ELISA-H00001731-M03-img0003.jpg "Sandwich ELISA: Septin-1 Antibody (1F12) [H00001731-M03] - Detection limit for recombinant GST tagged SEPT1 is 3 ng/ml as a capture antibody.")
