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Recombinant Mouse Cadherin-11 Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Cadherin-11 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of CCRT cotton rat osteosarcoma cells. The ED50 for this effect is 0.35-1.4 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse Cadherin-11 protein
Mouse Cadherin-11
(Met1 - Thr617)
Accession # P55288		 IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe23, Leu25, & Gly54
Protein/Peptide Type
Recombinant Proteins
Gene
Cdh11
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
89.1 kDa (mature protein, monomer); 92.8 kDa (pro-protein, monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-110 kDa, reducing conditions
Publications
Read Publication using
6627-CA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Cadherin-11 Fc Chimera Protein, CF

  • CAD11OSF-4
  • cadherin 11, type 2, OB-cadherin (osteoblast)
  • Cadherin11
  • Cadherin-11
  • CDH11
  • CDHOB
  • OB
  • OB-Cadherin
  • Osteoblast cadherin

Background

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2 ‑ 5). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (6). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (7, 8). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (7 ‑ 9). In the synovium, Cadherin-11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (10, 11). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (12). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (13). Mature mouse Cadherin-11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (2, 3, 14). Within the ECD, mouse Cadherin-11 shares 97% and 98% aa sequence identity with human and rat Cadherin-11, respectively. An 80 kDa portion of the Cadherin-11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (4, 15).
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kimura, Y. et al. (1995) Dev. Biol. 169:347.
  3. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337.
  4. McCusker, C. et al. (2009) Mol. Biol. Cell 20:78.
  5. Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909.
  6. Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840.
  7. Tamura, D. et al. (2008) Int. J. Oncol. 33:17.
  8. Chu, K. et al. (2008) Mol. Cancer Res. 6:1259.
  9. Huang, C.-F. et al. (2010) Cancer Res. 70:4580.
  10. Valencia, X. et al. (2004) J. Exp. Med. 200:1673.
  11. Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305.
  12. Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581.
  13. Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061.
  14. Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092.
  15. Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.

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Publications for Cadherin-11 (6627-CA)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Cdh11
Uniprot