RBFOX3/NeuN Antibody (1B7) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Rbfox3 (also known as Fox-3, Fox1 homolog C, Hrnbp3, and D11Bwg0517e) is one of three mammalian members of the RNA-binding protein Rbfox gene family, all of which are involved in regulating alternative RNA splicing. Originally identified as an epitope, NeuN is located within the N-terminal region (6-15 amino acids) of Rbfox3. Rbfox family members are highly conserved, having a single RNA recognition motif (RRM)-type RNA binding domain (RBD) near the center of the protein sequence. The RBD amino acid (aa) sequence is identical between members, Rbfox1 and Rbfox2, with 4 differences within the 77 aa domain of Rbfox3. The fox3 gene is comprised of 15 exons in humans, 11 exons in rat, with 3 variants in mouse containing 14 exons and another 3 variants containing 15 exons. The Rbfox3 protein is highly conserved across human, mouse, and rat with 98.9% sequence identity between mouse (isoform I) and rat and 83.9% sequence identity between mouse (isoform I) and human. The expression of Rbfox3/NeuN is restricted to the nervous system and has widely been used in stroke research. It is recognized as a marker of mature neuronal cell types in the spinal cord, cerebral cortex, hippocampus, dorsal thalamus, caudate/putamen, and cerebellum. NeuN has been shown to bind DNA and is predominantly nuclear. Differences in immunoreactivity have been reported between Rbfox3/NeuN subtypes in which the 46kDa is mainly found in the nucleus whereas the 48kDa form is primarily distributed in the cytoplasm (1,2).

Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).

References

1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.

2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: FLOW, ICC/IF.

Showing Publications 1 - 7 of 7.

Publications using NBP1-92693AF647	Applications	Species
Bryant A, Li Z, Jayakumar R et al. Endothelial Cells Are Heterogeneous in Different Brain Regions and Are Dramatically Altered in Alzheimer's Disease The Journal of Neuroscience 2023-06-14 [PMID: 37208174]
Bryant A, Li Z, Jayakumar R et al. Endothelial Cells are Heterogeneous in Different Brain Regions and are Dramatically Altered in Alzheimer's Disease bioRxiv : the preprint server for biology 2023-02-16 [PMID: 36824974] (ICC/IF, Human)	ICC/IF	Human
Astrid Wachter, Maya E. Woodbury, Sylvia Lombardo, Aicha Abdourahman, Carolin Wuest, Emily McGlame, Timothy Pastika, Joseph Tamm, Nandini Romanul, Kiran Yanamandra, Rachel Bennett, Gen Lin, Taekyung Kwon, Fan Liao, Corinna Klein, Yelena Grinberg, Methasit Jaisa-aad, Huan Li, Matthew. P. Frosch, Markus P. Kummer, Sudeshna Das, Tammy Dellovade, Eric H. Karran, Xavier Langlois, Janina S. Ried, Alberto Serrano-Pozo, Robert V. Talanian, Knut Biber, Bradley T. Hyman Landscape of brain myeloid cell transcriptome along the spatiotemporal progression of Alzheimer’s disease reveals distinct sequential responses to A beta and tau Acta Neuropathologica 2024-04-01 [PMID: 38557897]
Gulen MF, Samson N, Keller A et al. cGAS-STING drives ageing-related inflammation and neurodegeneration Nature 2023-08-01 [PMID: 37532932] (Mouse) Details: scRNA-seq		Mouse
Puvogel S, Alsema A, Kracht L et al. Single-nucleus RNA sequencing of the midbrain blood-brain barrier cells in schizophrenia reveals subtle transcriptional changes with overall preservation of cellular proportions and phenotypes Research Square 2022-06-23 [PMID: 36192459] (FLOW, Human)	FLOW	Human
Gerrits E, Giannini LAA, Brouwer N Et al. Neurovascular dysfunction in GRN-associated frontotemporal dementia identified by single-nucleus RNA sequencing of human cerebral cortex Nat Neurosci 2022-07-25 [PMID: 35879464] (FLOW, Human) Details: Citation using the Alexa Fluor 647 version of this antibody.	FLOW	Human

Array NBP1-92693AF647

• RBFOX3/NeuN Antibodies
• RBFOX3/NeuN Lysate
• RBFOX3/NeuN Proteins